Antibacterial activity of honey on staphylococcus aureus escherichia coli and streptococcus pyogen isolated from wound | Blazingprojects Postgraduate Thesis
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Antibacterial activity of honey on staphylococcus aureus escherichia coli and streptococcus pyogen isolated from wound

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Antibacterial Activity
  • 2.2Properties of Honey Relevant to Antibacterial Activity
  • 2.3Staphylococcus aureus: Characteristics and Antibiotic Resistance
  • 2.4Escherichia coli: Characteristics and Pathogenicity
  • 2.5Streptococcus pyogen: Pathogenicity and Antibiotic Resistance
  • 2.6Previous Studies on Honey's Antibacterial Activity
  • 2.7Comparison with Other Natural Antibacterial Agents
  • 2.8Mechanisms of Honey's Antibacterial Action
  • 2.9Bioactive Components in Honey
  • 2.10Future Research Directions

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Design and Methodology
  • 3.2Selection of Honey Samples
  • 3.3Isolation and Culturing of Bacterial Strains
  • 3.4Antibacterial Susceptibility Testing Methods
  • 3.5Experimental Setup for Antibacterial Testing
  • 3.6Data Collection and Analysis
  • 3.7Ethical Considerations
  • 3.8Statistical Analysis Techniques

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Overview of Research Findings
  • 4.2Antibacterial Activity of Different Honey Samples
  • 4.3Comparison of Antibacterial Efficacy on Staphylococcus aureus
  • 4.4Comparison of Antibacterial Efficacy on Escherichia coli
  • 4.5Comparison of Antibacterial Efficacy on Streptococcus pyogen
  • 4.6Relationship between Honey Properties and Antibacterial Activity
  • 4.7Discussion on Mechanisms of Action
  • 4.8Implications for Wound Care

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Findings
  • 5.2Conclusion
  • 5.3Recommendations for Further Research
  • 5.4Practical Applications and Future Implications
  • 5.5Reflection on Research Process

Thesis Abstract

Antibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus, Escherichia coli and Streptococcus pyogens isolated from wound was studied. Agar well diffusion method was used to determine the antibacterial activity of the honey on the test microorganisms. The result revealed that the two honey samples have heavy antibacterial activities against the test organisms and zones of inhibition were obtained showing high antibacterial activity. The antibacterial activity increased with increase in the concentrations and honey from Nsukka produced a high antibacterial activity (clearer zone) on staphylococcus aureus and Escherichia coli at all concentration and moderately for streptococcus pyogens. The use of honey as a therapeutic substance has been rediscovered by the medical profession on more recent times, and it is gaining acceptance as an antibacterial agent for the treatment of ulcers and bed sores, and other infections resulting from burns and wounds.

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