Application of Next-Generation Sequencing in Molecular Diagnosis of Infectious Diseases

Thesis Abstract

Abstract
Next-generation sequencing (NGS) has revolutionized the field of medical laboratory science, particularly in the molecular diagnosis of infectious diseases. This thesis explores the application of NGS technologies in improving the accuracy, speed, and efficiency of diagnosing infectious diseases caused by various pathogens. The research delves into the background of NGS, highlighting its significance in the context of infectious disease diagnosis and management. The problem statement addresses the limitations of traditional diagnostic methods in accurately identifying infectious agents and the need for more advanced approaches to enhance diagnostic precision. The objective of this study is to evaluate the effectiveness of NGS in diagnosing infectious diseases and to assess its potential for routine clinical use. The literature review encompasses ten key areas, including the principles of NGS technology, its applications in infectious disease diagnosis, comparative analysis with conventional methods, and the challenges and opportunities associated with its implementation in clinical settings. The review also discusses recent studies and advancements in the field, providing a comprehensive overview of the current state of NGS in infectious disease diagnostics. In the research methodology section, detailed information is provided on the experimental design, sample collection and preparation, sequencing protocols, data analysis techniques, and quality control measures employed to ensure the reliability and validity of the study results. The methodology also includes a description of the study population and the specific infectious diseases targeted in the analysis. The discussion of findings chapter presents a detailed analysis of the results obtained from the NGS-based diagnostic approach, comparing them with traditional methods and highlighting the advantages and limitations of NGS in identifying infectious pathogens. The chapter also discusses the implications of the findings for clinical practice and future research directions in the field of infectious disease diagnostics. In conclusion, this thesis demonstrates the potential of NGS as a powerful tool in the molecular diagnosis of infectious diseases, offering rapid and accurate identification of pathogens. The study underscores the importance of integrating NGS into routine clinical practice to improve patient outcomes and public health interventions. Overall, the findings support the adoption of NGS technology as a valuable asset in the fight against infectious diseases. Keywords Next-generation sequencing, molecular diagnosis, infectious diseases, medical laboratory science, diagnostic accuracy.