Monostaining of kidney and liver tissues with extracts of tumeric (curcuma longa) and zobo (hibiscus sabdariffa)
Table Of Contents
Chapter ONE
INTRODUCTION
- 1.1Introduction
- 1.2Background of Study
- 1.3Problem Statement
- 1.4Objective of Study
- 1.5Limitation of Study
- 1.6Scope of Study
- 1.7Significance of Study
- 1.8Structure of the Research
- 1.9Definition of Terms
Chapter TWO
LITERATURE REVIEW
- 2.1Overview of Literature Review
- 2.2Historical Perspective
- 2.3Theoretical Framework
- 2.4Previous Studies on Monostaining
- 2.5Benefits of Monostaining
- 2.6Challenges of Monostaining
- 2.7Monostaining Techniques
- 2.8Comparison of Different Staining Methods
- 2.9Applications of Monostaining
- 2.10Future Trends in Monostaining Research
Chapter THREE
RESEARCH METHODOLOGY
- 3.1Research Methodology Overview
- 3.2Research Design
- 3.3Sampling Techniques
- 3.4Data Collection Methods
- 3.5Data Analysis Procedures
- 3.6Ethical Considerations
- 3.7Validity and Reliability
- 3.8Limitations of the Methodology
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- 4.1Summary of Findings
- 4.2Analysis of Kidney Tissue Staining Results
- 4.3Analysis of Liver Tissue Staining Results
- 4.4Comparison of Turmeric and Zobo Extracts
- 4.5Interpretation of Results
- 4.6Discussion on Research Hypotheses
- 4.7Implications of Findings
- 4.8Recommendations for Further Research
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- 5.1Conclusion and Summary
- 5.2Recap of Research Objectives
- 5.3Key Findings Review
- 5.4Contributions to the Field
- 5.5Practical Implications
- 5.6Suggestions for Future Research
Thesis Abstract
Monstaining is a special kind of staining procedure that highlights single cells. Most stains used in histopathology are synthetic, toxic and costly for use hence this study was carried out at the histopathology laboratory of Imo state university to research more on the probability of replacing routine toxic and costly stains for natural, non toxic, less costly and effective stains that can highlight observably single cellular structures when viewed microscopically. Twelve sections were used for this study (6 liver and 6 kidney sections), obtained from the federal medical center Owerri of which was divided into four groups of liver and kidney sections respectively. This division was according to stains to be used on both sections either with haematoxylin and eosin or prepared adjusted PH extracts of zobo and turmeric viz The group 1 (3 liver sections) were stained with haematoxylin and eosin, group 2 (3liver sections) stained with zobo and turmeric plant extracts, group 3 (3kidney sections) stained with haematoxylin and eosin stains and group 4 (3kidney sections) stained with zobo and turmeric plant extracts. After analysis it was concluded that zobo and turmeric were not very good stains in highlighting single cell structures when compared to routinely used haematoxylin and eosin stains.
Thesis Overview
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