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Isolation and identification of bacteria from food vendors and some vegetable available at ogbete market enugu.

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Bacteria
2.2 Foodborne Pathogens
2.3 Sources of Contamination in Food
2.4 Food Safety Regulations
2.5 Microbiological Testing Methods
2.6 Bacterial Identification Techniques
2.7 Health Implications of Bacterial Contamination
2.8 Previous Studies on Bacterial Contamination
2.9 Impact of Bacterial Contamination on Public Health
2.10 Strategies for Bacterial Control in Food

Chapter THREE

3.1 Research Design
3.2 Sampling Techniques
3.3 Data Collection Methods
3.4 Data Analysis Procedures
3.5 Research Instruments
3.6 Research Validity and Reliability
3.7 Ethical Considerations
3.8 Limitations of the Research Methodology

Chapter FOUR

4.1 Overview of Research Findings
4.2 Identification of Bacterial Strains
4.3 Distribution of Bacteria in Food Vendors
4.4 Comparison of Bacterial Contamination in Different Foods
4.5 Factors Contributing to Bacterial Contamination
4.6 Public Health Implications
4.7 Recommendations for Mitigating Bacterial Contamination
4.8 Future Research Directions

Chapter FIVE

5.1 Summary of Findings
5.2 Conclusions
5.3 Implications for Public Health
5.4 Recommendations
5.5 Contribution to Existing Knowledge

Thesis Abstract

Safety of food is a basic requirement of food quality. A total of 25 street food samples (Jollof rice, egwusi soup, ugu, water leaf and green), were randomly purchased from five different vendors in Ogbete main market Enugu. The samples were transported in ice to the laboratory. The samples were bacteriologically analyzed using pour plate technique and sub-culture. Pour plate techniques was done by carrying out serial dilution of the sample after which the first tube and the last tube were picked and 1ml of each sample was pipette into a Nutrient agar, the plate was then incubated for 24 hours at 37oC after which the plate were examined for growth. Sub culture was done using bacteriological agar. All the screened food samples had varying levels of bacterial growth ranging from 1.0 X 105 to 3.0 X 106 cfu/ml. ninety percent of the sampled foods had bacterial counts above the acceptable limits (104 cfu/ml) and 10% of the samples had bacterial counts less than (<104 cfu/ml). Six bacterial species were isolated from the foods sampled. Staphylococcus arueus, Bacillus cereus, Vibrio spp, Salmonella spp, Escherichia coli and Shigella spp. More than one pathogenic micro organism were isolated from jollof rice and water leaf. The findings revealed that street foods are potential vehicles for transmitting food borne illnesses thus the need to develop practical strategies geared toward street food safety.

Thesis Overview

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