Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNA molecules that play crucial roles in regulating gene expression at the post-transcriptional level. In the context of cancer, dysregulation of miRNAs has been implicated in tumorigenesis, tumor progression, and metastasis. This thesis aims to explore the specific role of miRNAs in regulating gene expression in cancer cells, with a focus on understanding the underlying mechanisms and potential therapeutic implications. The introduction provides a comprehensive overview of miRNAs, cancer biology, and the significance of studying miRNA-mediated gene regulation in cancer cells. The background of the study delves into the current understanding of miRNA dysregulation in cancer and highlights the need for further research in this area. The problem statement identifies the gaps in knowledge regarding the specific mechanisms through which miRNAs regulate gene expression in cancer cells. The objectives of the study include investigating the role of specific miRNAs in cancer progression, identifying target genes regulated by these miRNAs, and elucidating the signaling pathways involved in miRNA-mediated gene regulation. The limitations of the study are discussed, along with the scope of the research, which focuses on in vitro and in vivo experiments using cancer cell lines and animal models. The significance of the study lies in its potential to uncover novel therapeutic targets for cancer treatment based on miRNA-mediated gene regulation mechanisms. The structure of the thesis is outlined to guide the reader through the various chapters, including the literature review, research methodology, findings discussion, and conclusion. The literature review synthesizes existing research on miRNA dysregulation in cancer, highlighting key studies that have elucidated the role of miRNAs in cancer progression. The research methodology section details the experimental approaches used to investigate miRNA-mediated gene regulation in cancer cells, including cell culture techniques, molecular biology assays, and bioinformatics analysis. The findings discussion chapter presents the results of the experiments, including the identification of specific miRNAs and their target genes involved in cancer cell proliferation, migration, and invasion. The implications of these findings for cancer therapy are discussed in the context of developing miRNA-based therapeutics. In conclusion, this thesis provides valuable insights into the role of miRNAs in regulating gene expression in cancer cells, shedding light on the complex interplay between miRNAs, target genes, and signaling pathways in cancer progression. The potential for targeting miRNAs as a novel therapeutic strategy for cancer treatment is highlighted, paving the way for future research in this exciting field of study.

Thesis Overview

The project titled "Exploring the role of microRNAs in regulating gene expression in cancer cells" aims to investigate the intricate mechanisms by which microRNAs influence gene expression in cancer cells. MicroRNAs are small non-coding RNA molecules that play crucial roles in post-transcriptional gene regulation by binding to specific messenger RNA (mRNA) targets, thereby modulating their stability and translation. In the context of cancer, dysregulation of microRNAs has been implicated in various aspects of tumorigenesis, including cell proliferation, differentiation, apoptosis, and metastasis. This research will delve into the specific microRNAs that are known to be dysregulated in different types of cancer and explore how these molecules interact with their target mRNAs to influence gene expression patterns. By elucidating the molecular pathways through which microRNAs contribute to the development and progression of cancer, this study aims to provide valuable insights into potential therapeutic targets for cancer treatment. The project will employ a multidisciplinary approach, combining bioinformatics analyses, cell culture experiments, and molecular biology techniques to investigate the functional roles of specific microRNAs in regulating gene expression in cancer cells. Through a comprehensive examination of the underlying mechanisms involved, this research seeks to uncover novel biomarkers and therapeutic strategies that could ultimately improve the diagnosis and treatment of cancer. Overall, this project holds the potential to advance our understanding of the complex interplay between microRNAs and gene expression in cancer cells, offering new avenues for targeted interventions and personalized medicine in the fight against cancer.