Home / Biochemistry / The use of bitterleaf (vernonia amygdalina) extract as a means of extending the shelf-life of locally brewed sorghum beer

The use of bitterleaf (vernonia amygdalina) extract as a means of extending the shelf-life of locally brewed sorghum beer

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Locally Brewed Sorghum Beer
2.2 Properties of Bitterleaf (Vernonia amygdalina)
2.3 Shelf-life of Beverages
2.4 Impact of Microorganisms on Shelf-life
2.5 Traditional Preservation Methods
2.6 Previous Studies on Shelf-life Extension
2.7 Chemical Composition of Bitterleaf Extract
2.8 Mechanisms of Shelf-life Extension
2.9 Consumer Perception of Natural Preservatives
2.10 Regulatory Considerations

Chapter THREE

3.1 Research Design
3.2 Sampling Techniques
3.3 Data Collection Methods
3.4 Experimental Setup
3.5 Data Analysis Procedures
3.6 Ethical Considerations
3.7 Validity and Reliability
3.8 Limitations of the Methodology

Chapter FOUR

4.1 Analysis of Shelf-life Extension Results
4.2 Comparison with Traditional Preservatives
4.3 Sensory Evaluation of Treated Beer
4.4 Microbiological Analysis of Samples
4.5 Chemical Analysis of Treated Beer
4.6 Consumer Acceptance Studies
4.7 Economic Viability Assessment
4.8 Environmental Impact Considerations

Chapter FIVE

5.1 Summary of Findings
5.2 Conclusions
5.3 Implications for Future Research
5.4 Recommendations for Industry
5.5 Contribution to Knowledge

Thesis Abstract

Locally brewed beer (sorghum beer) is an indigenous alcohol average. It is produced mainly from sorghum grain (Sorghum vulgare ) and the process of brewing involves malting, mashing, souring, fermentation and packaging. Locally brewed beer without hops has a short shelf-life. The aqueous extract of a tropical plant, bitter-leaf was used as the hop .Two type of drink were produced, one with hops and another without hops. Chemical analyses were carried out on both drinks, which gave a significant (p≤0.05) difference. Sorghum beer with hops has a total acidity of 0.562±0.03, fixed acidity of 0.203±0.001, Volatile acidity 0.360±0.02, PH 3.93, specific gravity 1.042±0.003, total dissolved solids 1.12×105ppm, total suspended solids 1.65×105ppm, ethanol content of 3.43±0.03 respectively. While sorghum beer without hops has a total acidity of 0.652 ±0.002, fixed acidity of 0.176±0.002, volatile acidity of 0.476±0.03, PH of 3.50 specific gravity of 1.021±0.003, total dissolved solid of 1.15×105ppm, total suspended solids of 1.5×105ppm and an ethanol content of 3.65±0.02. The microbial count for sorghum beer with hop had no growth of E.coli, streptococcus and staphylococcus but aspergillus was present from the 4th day and heaviest on the 8th day. While the drink without hop had growth of staphylococcus and aspergillus which was seen from the 2nd day and heaviest on the 4th day but E.coli and streptococcus was absent. From the results, the drink with hops could keep for a longer period of time than the other drink without hops. Thus, the aqueous extract of Vernonia amygdalina could extend the shelf-life of locally brewed sorghum beer.

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