<p> </p><p><strong>INTRODUCTION AND LITERATURE REVIEW</strong></p><p><strong>

Chapter ONE

</strong>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; </p><p>1.0 &nbsp; INTRODUCTION AND LITERATURE REVIEW &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; </p><p>1.1 &nbsp; &nbsp; &nbsp; &nbsp; Occurrence and Distribution</p><p>1.2 &nbsp; &nbsp; &nbsp; &nbsp; Assay Method</p><p>1.3 &nbsp; &nbsp; &nbsp; Methods of Purification</p><p>1.4 &nbsp; &nbsp; &nbsp; Structural properties of rhodanese</p><p>1.4.1 &nbsp; &nbsp; &nbsp; &nbsp;Molecular Weight</p><p>1.4.2 &nbsp; &nbsp; &nbsp; Amino Acid Composition</p><p>1.4.3 &nbsp; &nbsp; &nbsp; Amino Acid Sequence</p><p>1.4.4 &nbsp; &nbsp; Tertiary Structure of Rhodanese</p><p>1.4.5 &nbsp; &nbsp; &nbsp; Active site of Rhodanese</p><p>1.4.6 &nbsp; &nbsp; Mechanism of Action of rhodanese</p><p>1.5 &nbsp; &nbsp; &nbsp; Physical and Catalytic properties of rhodanese</p><p>1.5.1 &nbsp; Isoelectric point</p><p>1.5.2 &nbsp; &nbsp; Effect of pH</p><p>1.5.3 &nbsp; &nbsp; Effect of Temperature</p><p>1.5.4 &nbsp; &nbsp; &nbsp; Substrate specificity</p><p>1.5.5 &nbsp; &nbsp; Turnover number</p><p>1.5.6 &nbsp; &nbsp; Inhibition</p><p>1.5.7 &nbsp; &nbsp; Effect of Sulphyldryl reagent</p><p>1.6 &nbsp; &nbsp; &nbsp;Cloning of rhodanese gene</p><p>1.7 &nbsp; &nbsp; Biological importance of rhodanese</p><p>1.8 &nbsp; &nbsp; Background information</p><p>1.9 &nbsp; &nbsp; Specific Aims of research</p><p>1.10 &nbsp; Justification of research</p><p>

Chapter TWO

</p><p>2.0 &nbsp; &nbsp; &nbsp; &nbsp;MATERIALS AND METHODS &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; </p><p>2.1 &nbsp; &nbsp; &nbsp; &nbsp; Materials</p><p>2.2 &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;Methods</p><p>2.2.1 &nbsp; &nbsp; &nbsp; Preparation of Buffer and Reagents</p><p>2.2.1.1 &nbsp; Preparation of Borate buffer</p><p>2.2.1.2 &nbsp; Preparation of 15% formaldehyde from 40% formaldehyde</p><p>2.2.1.3 &nbsp; Preparation of sorbo reagent</p><p>2.2.1.4 &nbsp; Preparation of Bradford reagent</p><p>2.2.1.5 &nbsp; Preparation of 0.25M KCN</p><p>2.2.2 &nbsp; &nbsp; &nbsp; Enzyme source</p><p>2.2.3 &nbsp; &nbsp;Isolation and Purification of the enzyme</p><p>2.2.3.1 &nbsp; Homogenization</p><p>2.2.3.2 &nbsp; Ammonium sulphate precipitation</p><p>2.2.3.3 &nbsp; Reactive Blue-2-Agarose Affinity chromatography</p><p>2.2.4 &nbsp; &nbsp; Enzyme assay</p><p>2.2.5 &nbsp; &nbsp; &nbsp;Protein determination</p><p>2.2.6 &nbsp; &nbsp; &nbsp; Determination of kinetic parameters</p><p>2.2.7 &nbsp; &nbsp; Effect of pH on enzyme activity</p><p>2.2.8 &nbsp; &nbsp; Effect of Temperature on enzyme activity</p><p>2.2.9 &nbsp; &nbsp; Effect of Cations on enzyme</p><p>

Chapter THREE

</p><p>RESULTS &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</p><p>3.1 &nbsp; &nbsp; &nbsp; Purification of Rhodanese</p><p>3.2 &nbsp; &nbsp; &nbsp; Kinectic Parameters</p><p>3.3 &nbsp; &nbsp; &nbsp; Substrate Specificity</p><p>3.3.1 &nbsp; Effects of pH and temperature on enzyme activity</p><p>3.3.2 &nbsp; Effects of Cations</p><p><strong>

Chapter FOUR

</strong></p><p>4.0 &nbsp; &nbsp; &nbsp; &nbsp; Discussion and conclusion</p><p>4.1 &nbsp; &nbsp; &nbsp; &nbsp; Discussion</p><p>4.2 &nbsp; &nbsp; &nbsp; &nbsp; Conclusion</p><p>4.3 &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;Recommendation</p><p><strong>REFERENCES.</strong></p> <br><p></p>