Abstract                                                                                          

Chapter ONE

1.0             Introduction and Literature Review

1.1             Fibroid

1.1.1           Location and classification of fibroid

1.1.2           Pathogenesis and Pathophysiology

1.1.2.1       Pathogenesis

1.1.2.2       Pathophysiology

1.1.3           Signs, Symptoms and Causes of fibroid

1.2             Arginase

1.2.1           Role of arginase in human body

1.2.2           Aginase activities in Benign tumour

1.2.3           Arginase II expression in benign and malignant prostate tissue

1.3             Glutathione (GSH)

1.3.1           Role of glutathione as an antioxidant

1.3.2           Glutathione response to exercise

1.4             Sodium citrate

1.5             Aims and objectives

Chapter TWO

2.0             Materials and Methods

2.1             Materials

2.1.1           Apparatus

2.2             Methods

2.2.1           Preparation of buffers and reagents

2.3             Biochemical test

2.3.1           Arginase assay

2.3.2           Protein standard curve

2.3.3           Effect of Glutathione (GSH)

Chapter THREE

3.0             Results

Chapter FOUR

4.0             Discussion, Conclusion and Recommendation

4.1             Discussion

4.2             Conclusion

4.3           Recommendations

References

LIST OF FIGURES

Figure 1 –     Uterine Fibroid

Figure 2 –   Hydrolysis of L-arginine

Figure 3 –   Structure of arginase and the active site

Figure 4 –   Showing the effect of sodium citrate on arginase from human fibroid tissue

Figure 5 –   Showing the effect of Glutathione (GSH) on arginase from human fibroid tissue.