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Isolation and identification of microoragnisms in cassava mill effluent in the soil

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Microorganisms
2.2 Characteristics of Cassava Mill Effluent
2.3 Environmental Impact of Cassava Mill Effluent
2.4 Microbial Diversity in Soil
2.5 Interaction Between Microorganisms and Effluent
2.6 Previous Studies on Microorganisms in Cassava Mill Effluent
2.7 Methods for Isolation and Identification of Microorganisms
2.8 Role of Microorganisms in Soil Health
2.9 Adaptation of Microorganisms to Effluent
2.10 Future Research Directions on Microorganisms in Effluent

Chapter THREE

3.1 Research Methodology Overview
3.2 Sampling and Collection of Cassava Mill Effluent and Soil Samples
3.3 Isolation Techniques for Microorganisms
3.4 Identification Methods for Microorganisms
3.5 Data Analysis Procedures
3.6 Quality Control Measures
3.7 Ethical Considerations in Research
3.8 Statistical Analysis Methods

Chapter FOUR

4.1 Analysis of Microorganisms Isolated from Cassava Mill Effluent
4.2 Identification of Dominant Microbial Species
4.3 Diversity and Abundance of Microorganisms in Soil
4.4 Correlation Between Effluent Characteristics and Microbial Composition
4.5 Microbial Interactions and Ecosystem Functioning
4.6 Comparison with Previous Studies
4.7 Implications for Soil Health and Environmental Management
4.8 Recommendations for Future Research

Chapter FIVE

5.1 Conclusion and Summary of Findings
5.2 Key Insights from the Research
5.3 Contributions to Existing Knowledge
5.4 Practical Implications and Applications
5.5 Suggestions for Further Studies

Thesis Abstract

The Isolation and identification of microorganisms in cassava mill effluent in the soil in Orji, Imo State, Nigeria were carried out, soil samples were collected from site polluted with cassava mill effluent and also from adjacent site not polluted with cassava effluent to serve as control. The overall population of microorganisms in cassava mill effluent was determined. Isolation and characterization of succession of microorganism in cassava mill effluent was studied microbial count, Bacterial counts and fungal counts were also determined using a pour plate method. The microbial count ranges from 2.2 X 105 – 2.0 X 105 for THBC, 1.1 X 105 – 1.2 X 105 for TCC and the TFC ranges from 1.2 X 105-1.5 X 105. The bacteria isolated were Klebsiella spp., Escherichia coli , Pseudomonas spp., Staphylococcus aureus, and Streptococcus spp. while the fugal isolates include Penicillium spp., Aspergillus spp., mucor spp., and yeast cells Candida spp. the cassava mill effluent should be treated before discharge into the environment to prevent pollution.



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