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Isolation and characterization of antibiotic producing actinomycetes in a rhizosphere environment

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Actinomycetes
2.2 Antibiotic Production by Actinomycetes
2.3 Rhizosphere Environment
2.4 Interaction of Actinomycetes in the Rhizosphere
2.5 Isolation Techniques for Actinomycetes
2.6 Characterization Methods for Actinomycetes
2.7 Importance of Studying Antibiotic Producing Actinomycetes
2.8 Impact of Antibiotic Resistance on Actinomycetes
2.9 Role of Actinomycetes in Soil Health
2.10 Future Prospects in Actinomycetes Research

Chapter THREE

3.1 Research Design
3.2 Sampling Methods
3.3 Data Collection Techniques
3.4 Experimental Setup
3.5 Data Analysis Procedures
3.6 Ethical Considerations
3.7 Validity and Reliability
3.8 Research Limitations

Chapter FOUR

4.1 Overview of Research Findings
4.2 Isolation of Antibiotic Producing Actinomycetes
4.3 Characterization of Isolated Actinomycetes
4.4 Antibiotic Production Assays
4.5 Identification of Antibiotic Compounds
4.6 Interaction Studies in the Rhizosphere
4.7 Comparative Analysis of Antibiotic Activity
4.8 Implications of Findings

Chapter FIVE

5.1 Summary of Findings
5.2 Conclusion
5.3 Recommendations for Future Research
5.4 Practical Implications
5.5 Contributions to the Field

Thesis Abstract

Two hundred and forty isolates were isolated from the cow dung, compost, soils where the hides and skin of animals were burnt. Three isolates designated A1, A7, and A43 displayed clear zones of inhibition characteristic of Actinomycetes. The isolates were further characterized by examining their colony morphology, cover slip culture techniques, carbon utilization potentials, gram staining reaction and amylase activity. The α-amylase activity was determined on solid medium supplemented with starch. The detection α-amylase activity of the isolates was based on the formation of clear zones around the colonies when flooded with iodine. The decolourization abilities of the isolates were also determined. The isolates showed good absorption characteristics and degrading capacity on the dye Congo red and brilliant green. It showed that Actinomycetes are potential decolorizer of dye stuffs and are good biosorbent.

Thesis Overview

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