Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Protein and Iron Metabolism
2.2 Malaria Parasite (Plasmodium berghei, berghei)
2.3 Tetracycline: Mechanism of Action
2.4 Previous Studies on Protein and Iron Levels in Mice
2.5 Effects of Malaria Infection on Protein and Iron Levels
2.6 Impact of Tetracycline Treatment on Protein and Iron Levels
2.7 Relationship Between Protein and Iron Levels in Infected Mice
2.8 Factors Affecting Protein and Iron Assay Accuracy
2.9 Methods for Estimating Total Serum Protein and Iron
2.10 Role of Protein and Iron in Immune Response

Chapter THREE

3.1 Research Design and Rationale
3.2 Sampling Methods and Population Selection
3.3 Data Collection Techniques
3.4 Experimental Procedures and Treatments
3.5 Measurement of Total Serum Protein Levels
3.6 Measurement of Total Serum Iron Levels
3.7 Statistical Analysis Methods
3.8 Ethical Considerations and Animal Welfare

Chapter FOUR

4.1 Presentation of Research Findings
4.2 Analysis of Protein and Iron Levels in Infected Mice
4.3 Comparison of Protein and Iron Levels pre and post-Treatment
4.4 Correlation Between Protein and Iron Levels
4.5 Effects of Tetracycline on Protein and Iron Levels
4.6 Discussion on Variability in Protein and Iron Assays
4.7 Interpretation of Results in the Context of Immune Response
4.8 Implications for Future Research

Chapter FIVE

5.1 Conclusion and Summary of Findings
5.2 Recap of Research Objectives
5.3 Contributions to the Field of Study
5.4 Practical Applications and Future Directions
5.5 Recommendations for Further Research