Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNA molecules that play crucial roles in post-transcriptional gene regulation. In cancer cells, dysregulation of miRNAs can lead to aberrant gene expression patterns, contributing to tumorigenesis and cancer progression. This thesis aims to investigate the specific roles of miRNAs in regulating gene expression in cancer cells, with a focus on their potential as therapeutic targets or biomarkers. Chapter 1 provides an introduction to the study, presenting the background information on miRNAs, the problem statement, objectives, limitations, scope, significance of the study, and the structure of the thesis. The definitions of key terms related to miRNAs and cancer biology are also provided to establish a foundational understanding for the subsequent chapters. Chapter 2 consists of a comprehensive literature review that explores existing research on miRNAs in cancer cells. This chapter covers ten key aspects, including the general functions of miRNAs, their dysregulation in cancer, the mechanisms of miRNA-mediated gene regulation, and the potential clinical applications of miRNAs in cancer diagnosis and therapy. In Chapter 3, the research methodology is detailed, outlining the experimental approaches and techniques used to investigate the roles of miRNAs in regulating gene expression in cancer cells. This chapter includes descriptions of cell culture techniques, miRNA profiling methods, bioinformatics analysis, and functional assays to validate miRNA-target interactions. Chapter 4 presents the findings of the study, discussing the specific miRNAs identified as regulators of gene expression in cancer cells. The results of functional experiments and bioinformatics analyses are presented, highlighting the mechanisms through which these miRNAs modulate gene expression and impact cancer cell phenotypes. Finally, Chapter 5 provides a conclusion and summary of the thesis, discussing the implications of the findings in the context of cancer biology and potential therapeutic interventions. The limitations of the study are acknowledged, and future research directions are proposed to further elucidate the roles of miRNAs in cancer. In conclusion, this thesis contributes to the understanding of how miRNAs regulate gene expression in cancer cells and provides insights into the potential clinical applications of miRNAs as diagnostic biomarkers or therapeutic targets in cancer. Further research in this area is warranted to fully exploit the therapeutic potential of miRNAs in cancer treatment.

Thesis Overview

The project titled "Exploring the Role of MicroRNAs in Regulating Gene Expression in Cancer Cells" aims to investigate the intricate mechanisms by which microRNAs influence gene expression in cancer cells. MicroRNAs are small non-coding RNA molecules that play crucial roles in regulating gene expression by targeting specific messenger RNAs (mRNAs) for degradation or translational inhibition. In the context of cancer, dysregulation of microRNA expression has been linked to tumorigenesis, metastasis, and drug resistance. This research seeks to delve deeper into the specific microRNAs involved in regulating gene expression in various types of cancer cells. By identifying the key microRNAs and their target genes, this study aims to elucidate the underlying molecular pathways that contribute to cancer development and progression. Understanding the role of microRNAs in cancer gene regulation could provide valuable insights into potential therapeutic targets for cancer treatment. The research methodology will involve a combination of bioinformatics analysis, cell culture experiments, and molecular biology techniques to study the expression patterns of microRNAs and their target genes in cancer cells. By analyzing large-scale genomics data and conducting functional studies, this project aims to uncover novel regulatory networks controlled by microRNAs in cancer cells. Overall, this research project holds significant promise for advancing our understanding of the molecular mechanisms underlying cancer development and progression. By elucidating the role of microRNAs in regulating gene expression in cancer cells, this study may pave the way for the development of innovative therapeutic strategies targeting these regulatory pathways to combat cancer effectively.