Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNA molecules that play a crucial role in post-transcriptional gene regulation. In cancer cells, dysregulation of miRNAs has been implicated in the development and progression of various types of cancers. This study aimed to investigate the specific role of miRNAs in regulating gene expression in cancer cells, with a focus on understanding the underlying mechanisms and potential implications for cancer therapy. The research began with a comprehensive review of the literature on miRNAs and their involvement in cancer. The literature review highlighted the importance of miRNAs in cancer biology and their potential as diagnostic and therapeutic targets. The review also identified gaps in knowledge regarding the specific mechanisms by which miRNAs regulate gene expression in cancer cells. The research methodology employed in this study included both in vitro and in silico approaches. Cancer cell lines were used to investigate the expression levels of specific miRNAs and their target genes. In silico analysis was performed to predict potential miRNA-target interactions and elucidate the regulatory networks involved in cancer cell gene expression. The findings of this study revealed that several miRNAs were differentially expressed in cancer cells compared to normal cells. Furthermore, specific miRNAs were identified to target key genes involved in cancer progression, highlighting their potential as therapeutic targets. The in silico analysis provided insights into the complex regulatory networks controlled by miRNAs in cancer cells. The discussion of the findings delved into the implications of these results for cancer research and therapy. The study elucidated the role of miRNAs as critical regulators of gene expression in cancer cells and provided a deeper understanding of the mechanisms underlying their dysregulation in cancer. The potential for targeting specific miRNAs as a strategy for cancer treatment was also discussed. In conclusion, this research contributes to the growing body of knowledge on the role of miRNAs in cancer biology. The findings underscore the importance of miRNAs in regulating gene expression in cancer cells and suggest potential avenues for the development of novel therapeutic strategies targeting miRNAs. Further research in this area is warranted to fully exploit the therapeutic potential of miRNAs in cancer treatment.

Thesis Overview

The project "Investigating the role of miRNA in regulating gene expression in cancer cells" aims to delve into the intricate mechanisms through which microRNAs (miRNAs) influence gene expression in cancer cells. MicroRNAs are small non-coding RNA molecules that play crucial roles in post-transcriptional regulation of gene expression. In the context of cancer, dysregulation of miRNAs has been linked to tumorigenesis, metastasis, and resistance to therapy, making them attractive targets for therapeutic interventions. This research seeks to unravel the specific miRNAs involved in modulating gene expression in cancer cells and elucidate their downstream effects on cellular processes. By employing state-of-the-art molecular biology techniques, bioinformatics analyses, and cell culture experiments, the study aims to characterize the interactions between miRNAs and their target genes in cancer cells. Moreover, the project will investigate how dysregulated miRNA expression profiles contribute to the aberrant gene expression patterns observed in cancer. Through a comprehensive literature review, the research will synthesize existing knowledge on the role of miRNAs in cancer biology and highlight gaps in understanding that warrant further investigation. The methodology will involve the identification of differentially expressed miRNAs in cancer cell lines, validation of miRNA-target gene interactions, and functional assays to assess the impact of miRNA manipulation on cellular phenotypes. The findings of this study are expected to shed light on the underlying mechanisms by which miRNAs regulate gene expression in cancer cells and provide insights into potential therapeutic strategies targeting miRNAs for cancer treatment. By elucidating the intricate interplay between miRNAs and gene expression networks in cancer, this research holds promise for the development of novel diagnostic tools and therapeutic approaches to combat this complex and heterogeneous disease.