Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that play crucial roles in the regulation of gene expression at the post-transcriptional level. In cancer cells, dysregulation of miRNAs has been implicated in tumorigenesis, metastasis, and resistance to therapy. This thesis aimed to explore the intricate role of miRNAs in the regulation of gene expression in cancer cells, with a focus on understanding their impact on tumor development and progression. Chapter 1 provides an introduction to the study, presenting the background of miRNAs, the problem statement, research objectives, limitations, scope, significance, and the structure of the thesis. Definitions of key terms related to miRNAs and cancer biology are also provided. Chapter 2 comprises a comprehensive literature review that discusses ten key studies related to miRNAs in cancer. This section explores the current understanding of miRNA dysregulation in various types of cancer, highlighting the potential diagnostic, prognostic, and therapeutic implications of targeting miRNAs in cancer therapy. Chapter 3 details the research methodology employed in this study. It includes descriptions of the experimental design, cell culture techniques, miRNA profiling methods, bioinformatics analysis, and statistical approaches used to investigate the role of miRNAs in cancer cell gene expression regulation. Chapter 4 presents the findings of the study, offering an in-depth discussion of the data obtained from miRNA expression profiling and gene expression analysis in cancer cells. The results shed light on specific miRNAs that are dysregulated in cancer and their putative target genes involved in oncogenic pathways. In Chapter 5, the conclusion and summary of the thesis are provided. This section summarizes the key findings of the study and discusses their implications for understanding the molecular mechanisms underlying miRNA-mediated gene regulation in cancer cells. Future directions for research and potential clinical applications of targeting miRNAs in cancer therapy are also discussed. Overall, this thesis contributes to the growing body of knowledge on the role of miRNAs in cancer biology, providing valuable insights into the complex regulatory networks that govern gene expression in cancer cells. The findings of this study have the potential to advance our understanding of cancer pathogenesis and facilitate the development of novel therapeutic strategies targeting miRNAs for the treatment of cancer.

Thesis Overview

The project titled "Exploring the Role of MicroRNAs in Regulation of Gene Expression in Cancer Cells" aims to investigate the intricate mechanisms by which microRNAs modulate gene expression in cancer cells. MicroRNAs are small non-coding RNA molecules that play crucial roles in post-transcriptional gene regulation, thereby influencing various cellular processes, including cell proliferation, differentiation, and apoptosis. In cancer, dysregulation of microRNA expression has been implicated in tumor initiation, progression, and metastasis. The research will delve into the specific microRNAs involved in regulating gene expression in various types of cancer cells and explore their impact on key signaling pathways that drive tumorigenesis. By elucidating the interactions between microRNAs and target genes, the study aims to uncover novel insights into the molecular mechanisms underlying cancer development and progression. Through a comprehensive review of existing literature, the project will provide a detailed analysis of the current understanding of microRNA-mediated gene regulation in cancer. Additionally, experimental methodologies, including cell culture techniques, molecular biology assays, and bioinformatics analyses, will be employed to investigate the functional roles of specific microRNAs in cancer cell biology. The findings of this research have the potential to contribute to the development of innovative therapeutic strategies targeting microRNAs for cancer treatment. By gaining a deeper understanding of how microRNAs regulate gene expression in cancer cells, this study aims to pave the way for the identification of new biomarkers and therapeutic targets for precision oncology approaches. Overall, this research project seeks to advance our knowledge of the role of microRNAs in cancer progression and provide valuable insights into the molecular mechanisms driving tumorigenesis. Through a multidisciplinary approach combining biochemistry, molecular biology, and bioinformatics, the study aims to shed light on the complex interplay between microRNAs and gene expression in cancer cells, with the ultimate goal of improving diagnostics and treatment outcomes for cancer patients.