Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNA molecules that play crucial roles in post-transcriptional gene regulation. In cancer biology, dysregulation of miRNAs has been linked to the development and progression of various types of cancer. This thesis aims to explore the specific role of miRNAs in regulating gene expression in cancer cells, focusing on their potential as therapeutic targets and biomarkers. Chapter One provides an introduction to the study, presenting the background of miRNA research in cancer, the problem statement, objectives of the study, limitations, scope, significance, structure of the thesis, and key definitions of terms. The literature review in Chapter Two critically examines existing research on miRNAs and their involvement in cancer, highlighting key findings and gaps in knowledge. Chapter Three outlines the research methodology, detailing the experimental techniques and bioinformatics tools used to investigate miRNA-mediated gene regulation in cancer cells. This chapter includes information on sample collection, cell culture techniques, miRNA profiling, gene expression analysis, and statistical methods employed in the study. In Chapter Four, the findings of the research are discussed in detail, with a focus on the specific miRNAs identified as important regulators of gene expression in cancer cells. The role of these miRNAs in cancer progression, metastasis, drug resistance, and potential therapeutic applications are explored. The chapter also discusses the mechanisms by which miRNAs modulate gene expression and the impact of these regulatory networks on cancer biology. Chapter Five presents the conclusion and summary of the thesis, highlighting the key findings, implications, and future directions for research in this field. The potential clinical applications of miRNAs as diagnostic biomarkers and therapeutic targets in cancer are discussed, emphasizing the importance of further investigation to fully understand the complex regulatory networks involved. Overall, this thesis provides valuable insights into the role of miRNAs in regulating gene expression in cancer cells, shedding light on the potential mechanisms underlying cancer development and progression. The findings of this study contribute to the growing body of knowledge on miRNA biology and may have implications for the development of novel therapeutic strategies for cancer treatment.

Thesis Overview

Overview: The project titled "Exploring the role of microRNAs in regulating gene expression in cancer cells" aims to investigate the intricate mechanisms by which microRNAs influence gene expression within cancer cells. MicroRNAs are small non-coding RNA molecules that play crucial roles in post-transcriptional gene regulation. In cancer, dysregulation of microRNAs has been implicated in various processes such as tumor initiation, progression, and metastasis. Understanding how microRNAs modulate gene expression in cancer cells is essential for developing targeted therapeutic strategies to combat this complex disease. Chapter 1: Introduction The introduction sets the stage by providing an overview of microRNAs, cancer biology, and the significance of studying their role in gene expression regulation. It highlights the gaps in current knowledge and the rationale behind the research focus. Chapter 2: Literature Review This chapter critically examines existing literature on microRNAs and their impact on gene expression in cancer cells. It discusses key studies, methodologies, and findings to establish a comprehensive understanding of the subject matter. Chapter 3: Research Methodology The methodology section outlines the experimental approach and techniques used to investigate microRNA-mediated gene expression regulation in cancer cells. It includes details on cell culture, transfection methods, RNA isolation, microRNA profiling, and bioinformatics analysis. Chapter 4: Discussion of Findings In this chapter, the research findings are presented and discussed in detail. The data obtained from experimental analyses, including gene expression profiling and functional assays, are interpreted to elucidate the role of specific microRNAs in cancer cell biology. Chapter 5: Conclusion and Summary The final chapter provides a concise summary of the research outcomes and their implications for cancer biology. It discusses the significance of the findings, potential limitations, and future directions for further research in this field. Overall, this project aims to contribute to the growing body of knowledge on the regulatory functions of microRNAs in cancer cells. By unraveling the complex interplay between microRNAs and gene expression, this research has the potential to advance our understanding of cancer biology and pave the way for innovative therapeutic interventions targeting microRNA pathways in cancer treatment.