Thesis Abstract

Abstract
This thesis investigates the crucial role of microRNAs (miRNAs) in the regulation of gene expression in cancer cells. The dysregulation of miRNAs has been implicated in various aspects of cancer development and progression, making them attractive targets for cancer therapy. Chapter one provides an introduction to the study, highlighting the background information on miRNAs, the problem statement regarding their dysregulation in cancer, the objectives of the study, limitations, scope, significance, and the structure of the thesis. Additionally, key terms related to miRNAs and cancer are defined to aid in understanding the subsequent chapters. Chapter two presents a comprehensive literature review encompassing ten key areas related to miRNAs and cancer. The review synthesizes existing knowledge on the role of miRNAs in cancer progression, highlighting their potential as diagnostic biomarkers and therapeutic targets. Chapter three details the research methodology employed in this study, including the selection of cancer cell lines, miRNA profiling techniques, data analysis methods, and validation approaches. The chapter also discusses the ethical considerations and challenges faced during the research process. In chapter four, the findings of the study are extensively discussed, focusing on the identified miRNAs involved in regulating gene expression in cancer cells. The results shed light on the intricate mechanisms through which miRNAs modulate key signaling pathways implicated in cancer development, providing novel insights into potential therapeutic strategies. Lastly, chapter five presents the conclusion and summary of the thesis, summarizing the key findings, implications, and future directions for research in this field. The study underscores the importance of miRNAs as critical players in cancer pathogenesis and highlights the potential for targeting miRNAs in cancer therapy. Overall, this thesis contributes to the growing body of knowledge on the role of miRNAs in cancer and provides a foundation for further research in this area.

Thesis Overview

The project titled "Exploring the role of microRNAs in the regulation of gene expression in cancer cells" aims to investigate the intricate mechanisms by which microRNAs influence gene expression in cancer cells. MicroRNAs are small non-coding RNA molecules that play a crucial role in post-transcriptional gene regulation by targeting messenger RNA (mRNA) for degradation or translational repression. In cancer, dysregulation of microRNAs has been implicated in various processes such as cell proliferation, apoptosis, invasion, and metastasis. The research will delve into the specific microRNAs that are known to be dysregulated in different types of cancer and their impact on key signaling pathways involved in cancer progression. By elucidating the role of these microRNAs, the study aims to provide insights into how they contribute to the development and progression of cancer. The methodology will involve a combination of bioinformatics analysis, cell culture experiments, and molecular biology techniques to investigate the interactions between microRNAs and their target genes in cancer cells. By identifying the target genes of dysregulated microRNAs, the study seeks to unravel the underlying molecular mechanisms that drive cancer pathogenesis. The findings of this research are expected to contribute to a better understanding of the role of microRNAs in cancer biology and may have implications for the development of novel therapeutic strategies targeting these molecules. Ultimately, the project aims to advance our knowledge of the complex regulatory networks involved in cancer development and provide potential avenues for the development of targeted therapies for cancer treatment.