Thesis Abstract

Abstract
Cancer is a complex and multifaceted disease that continues to pose a significant challenge to human health worldwide. In recent years, microRNAs (miRNAs) have emerged as key regulators of gene expression and have been implicated in various aspects of cancer development and progression. This thesis aims to explore the role of miRNAs in cancer, focusing on their mechanisms of action, diagnostic potential, and therapeutic implications. Chapter One provides an introduction to the study, presenting the background of miRNAs in cancer research, defining the problem statement, objectives of the study, limitations, scope, significance, and the structure of the thesis. The chapter also includes definitions of key terms related to miRNAs and cancer. Chapter Two presents a comprehensive literature review on miRNAs in cancer, covering ten key areas such as the biogenesis of miRNAs, their role in cancer initiation and progression, and their potential as biomarkers and therapeutic targets. This chapter synthesizes existing knowledge and identifies gaps in current research. Chapter Three outlines the research methodology employed in this study. It includes detailed descriptions of the study design, sample collection and analysis, miRNA profiling techniques, bioinformatics analysis, and statistical methods. The chapter also discusses ethical considerations and potential biases. Chapter Four presents the findings of the study, including the identification of dysregulated miRNAs in various cancer types, their putative target genes, and the functional roles of these miRNAs in cancer development and progression. The chapter also discusses the implications of these findings for cancer diagnosis, prognosis, and treatment. Chapter Five concludes the thesis by summarizing the key findings, discussing their implications for future research and clinical practice, and highlighting the potential of miRNAs as novel targets for cancer therapy. The chapter also presents recommendations for further studies and discusses the limitations of the current research. Overall, this thesis provides a comprehensive overview of the role of miRNAs in cancer development and progression, highlighting their potential as diagnostic biomarkers and therapeutic targets. By elucidating the molecular mechanisms underlying miRNA-mediated oncogenesis, this study contributes to the growing body of knowledge aimed at improving cancer diagnosis and treatment strategies.

Thesis Overview

The research project titled "Exploring the role of microRNAs in cancer development and progression" aims to investigate the intricate involvement of microRNAs in the development and progression of cancer. MicroRNAs are small non-coding RNA molecules that play critical regulatory roles in gene expression and are implicated in various biological processes, including cancer development. Understanding the specific roles of microRNAs in cancer pathogenesis is essential for developing targeted therapeutic strategies and biomarkers for early detection and prognosis of different cancer types. The project will begin with a comprehensive literature review to explore the existing knowledge on microRNAs and their association with cancer. This review will highlight the current understanding of the mechanisms by which microRNAs contribute to tumorigenesis, metastasis, and resistance to therapy. By critically analyzing previous studies, the project aims to identify key microRNAs that are consistently dysregulated in various types of cancer and their functional implications in cancer biology. The research methodology will involve experimental approaches to investigate the expression profiles of selected microRNAs in cancer cell lines and clinical samples. State-of-the-art molecular biology techniques, such as quantitative real-time PCR and next-generation sequencing, will be employed to assess the expression levels of microRNAs and their target genes in different cancer models. Additionally, bioinformatics analysis will be utilized to predict potential target genes and signaling pathways regulated by dysregulated microRNAs in cancer. The findings of this project will be discussed in detail to elucidate the specific roles of identified microRNAs in promoting or inhibiting cancer progression. By correlating the expression patterns of microRNAs with clinical parameters, such as patient survival and response to therapy, the project aims to provide valuable insights into the diagnostic and prognostic relevance of microRNAs in cancer. Furthermore, the potential therapeutic implications of targeting dysregulated microRNAs in cancer treatment will be explored, with a focus on developing novel therapeutic strategies based on microRNA modulation. In conclusion, this research project on exploring the role of microRNAs in cancer development and progression represents a significant contribution to the field of cancer biology and personalized medicine. By unraveling the complex interplay between microRNAs and cancer pathogenesis, this study aims to advance our understanding of the molecular mechanisms underlying cancer and pave the way for the development of innovative diagnostic and therapeutic approaches for improving patient outcomes in oncology.