Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNA molecules that play a crucial role in regulating gene expression at the post-transcriptional level. In recent years, there has been growing interest in understanding the role of miRNAs in cancer biology, particularly their involvement in regulating gene expression in cancer cells. This thesis aims to analyze the role of miRNAs in regulating gene expression in cancer cells, with a focus on their potential implications for cancer diagnosis, prognosis, and therapy. Chapter One provides an introduction to the project, presenting the background of the study, problem statement, objectives, limitations, scope, significance, structure of the thesis, and definition of key terms. Chapter Two consists of a comprehensive literature review, covering ten key aspects related to miRNAs, cancer biology, and gene expression regulation in cancer cells. Chapter Three outlines the research methodology employed in this study, including sample collection, experimental techniques, data analysis methods, and statistical approaches. This chapter also discusses the ethical considerations and limitations of the research design. Chapter Four presents a detailed discussion of the findings obtained from the analysis of miRNA-mediated gene expression regulation in cancer cells. The chapter explores the specific miRNAs involved, their target genes, the mechanisms of action, and the potential implications for cancer treatment strategies. Finally, Chapter Five concludes the thesis by summarizing the key findings, discussing their significance in the context of cancer research, and highlighting potential future directions for further investigation. Overall, this thesis contributes to the growing body of knowledge on the role of miRNAs in cancer biology and provides valuable insights that could inform the development of novel therapeutic approaches for cancer treatment.

Thesis Overview

The project titled "Analysis of the role of microRNAs in regulating gene expression in cancer cells" aims to investigate the intricate mechanisms by which microRNAs influence gene expression in cancer cells. MicroRNAs are small non-coding RNA molecules that play a crucial role in post-transcriptional regulation of gene expression. In cancer, dysregulation of microRNAs can lead to abnormal expression of genes involved in cell proliferation, differentiation, and apoptosis, contributing to tumor development and progression. This research project will delve into the specific microRNAs implicated in various types of cancer and their target genes. By analyzing the interactions between microRNAs and their target mRNAs, the study seeks to elucidate how microRNAs modulate gene expression networks in cancer cells. Understanding these regulatory mechanisms is essential for identifying potential biomarkers for cancer diagnosis, prognosis, and therapeutic targets. The project will utilize a combination of bioinformatics tools, molecular biology techniques, and cell culture experiments to investigate the functional roles of specific microRNAs in cancer cells. By characterizing the expression profiles of microRNAs and their target genes, the research aims to uncover key pathways and signaling networks dysregulated in cancer. Furthermore, the project will explore the potential of targeting microRNAs as a novel therapeutic approach for cancer treatment. By modulating the expression of specific microRNAs, it may be possible to restore normal gene expression patterns and inhibit tumor growth. The findings from this study could pave the way for the development of innovative microRNA-based therapies for cancer patients. Overall, the research on the role of microRNAs in regulating gene expression in cancer cells is poised to advance our understanding of the molecular mechanisms underlying cancer development and progression. By elucidating the complex interplay between microRNAs and gene expression networks, this project holds promise for the identification of new diagnostic biomarkers and therapeutic strategies for cancer management.