Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Alpha-Amylase
2.2 Sources of Alpha-Amylase
2.3 Enzyme Production
2.4 Enzyme Purification Techniques
2.5 Enzyme Characterization Methods
2.6 Role of Alpha-Amylase in Biotechnology
2.7 Industrial Applications of Alpha-Amylase
2.8 Factors Affecting Alpha-Amylase Activity
2.9 Enzyme Immobilization Techniques
2.10 Comparison of Free and Immobilized Alpha-Amylase

Chapter THREE

3.1 Research Methodology Overview
3.2 Selection of Bacillus licheniformis Strain
3.3 Enzyme Extraction and Separation
3.4 Enzyme Purification Process
3.5 Immobilization Techniques Used
3.6 Characterization Methods Employed
3.7 Experimental Design and Setup
3.8 Data Collection and Analysis Methods

Chapter FOUR

4.1 Analysis of Enzyme Production Yield
4.2 Purification Efficiency Evaluation
4.3 Comparison of Free and Immobilized Enzyme Properties
4.4 Enzyme Stability Studies
4.5 Substrate Specificity Analysis
4.6 Effect of pH and Temperature on Enzyme Activity
4.7 Industrial Applicability Assessment
4.8 Discussion on Research Findings

Chapter FIVE

5.1 Summary of Research Findings
5.2 Conclusion and Implications
5.3 Recommendations for Future Research
5.4 Contribution to the Field
5.5 Closing Remarks