Home / Biochemistry / EFFECT OF METAL IONS ON ARGINASE FROM THE KIDNEY OF LOCAL GOAT (CAPRA AEGAGRUS HIRCUS)

EFFECT OF METAL IONS ON ARGINASE FROM THE KIDNEY OF LOCAL GOAT (CAPRA AEGAGRUS HIRCUS)

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Metal Ions
2.2 Arginase Enzyme Function
2.3 Interaction of Metal Ions with Enzymes
2.4 Goat Kidney Physiology
2.5 Previous Studies on Metal Ions and Enzymes
2.6 Effects of Metal Ions on Biological Systems
2.7 Regulation of Metal Ions in Organisms
2.8 Importance of Metal Ions in Biological Processes
2.9 Methods of Detecting Metal Ions in Biological Samples
2.10 Inhibition and Activation of Enzymes by Metal Ions

Chapter THREE

3.1 Research Methodology Overview
3.2 Study Design and Rationale
3.3 Sampling Techniques
3.4 Data Collection Methods
3.5 Data Analysis Procedures
3.6 Instrumentation and Materials Used
3.7 Ethical Considerations
3.8 Statistical Analysis Techniques

Chapter FOUR

4.1 Data Presentation and Analysis
4.2 Effects of Different Metal Ions on Arginase Activity
4.3 Comparison of Metal Ion Interactions with Arginase
4.4 Relationship Between Metal Ion Concentration and Enzyme Inhibition
4.5 Factors Influencing Metal Ion Binding to Arginase
4.6 Interpretation of Experimental Results
4.7 Discussion on the Implications of Metal Ions on Enzyme Function
4.8 Comparison with Previous Studies

Chapter FIVE

5.1 Summary of Findings
5.2 Conclusion and Recommendations
5.3 Contributions to Existing Knowledge
5.4 Implications for Future Research
5.5 Reflections on the Research Process

Thesis Abstract

This research is aimed at isolating, partially purifying and determination of arginase from the kidney of a local goat. Methods used in the isolation and partial purification of the enzyme include; Ammonium sulphate precipitation, dialysis.etc. Result from this work showed that arginase isolated from a local goat has a specific activity of 0.358 with a yield of 12.12% incubation of the enzyme with different metal ions shows that the enzyme is inhibited by Nacl and Bacl2.



Thesis Overview

1.0     Introduction and Literature review

1.1     Introduction

1.2     Literature review

1.2.1 History of arginase

1.3     Occurrence and distribution of arginase

1.3.1 Bacteria

1.3.2 Fungi

1.3.3 Lichens

1.3.4 Yeast

1.3.5 Insects

1.4     The substrate: Arginase

1.5     Scientific classification of local goat

1.6     Aim and Objectives


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