1. Introduction
1.1 Background and significance
1.2 Objectives of the project
2. RNA interference: An overview
2.1 Definition and historical background
2.2 Mechanisms of RNA interference
3. Small interfering RNAs (siRNAs)
3.1 Biogenesis of siRNAs
3.2 Roles of siRNAs in gene silencing
4. MicroRNAs (miRNAs)
4.1 Biogenesis of miRNAs
4.2 Functions of miRNAs in gene regulation
5. RNA-induced silencing complex (RISC)
5.1 Assembly and composition of RISC
5.2 Mechanisms of target mRNA recognition and degradation
This project aims to study the mechanisms of RNA interference (RNAi), a highly conserved biological process that regulates gene expression through the degradation or translational repression of target mRNAs. The project will focus on understanding the molecular machinery involved in RNAi, including small interfering RNAs (siRNAs), microRNAs (miRNAs), and the RNA-induced silencing complex (RISC). Experimental techniques such as RNA sequencing, gene knockdown, and bioinformatics analysis will be employed to investigate the roles of RNAi in various biological processes, including development, immunity, and disease. The findings from this project will contribute to our understanding of gene regulation and may have implications in therapeutic applications.
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