Studying the mechanisms of RNA interference
Table Of Contents
1. Introduction
1.1 Background
1.2 Objectives
2. RNA Interference: An Overview
2.1 Definition and History
2.2 Biogenesis of siRNAs and miRNAs
2.3 Mechanisms of Gene Silencing
3. Techniques for Studying RNAi
3.1 RNAi Knockdown and Overexpression
3.2 RNAi Screening
3.3 Bioinformatics Approaches
4. Applications of RNAi
4.1 Therapeutic Applications
4.2 Functional Genomics
4.3 Agriculture and Pest Control
5. Challenges and Future Directions
5.1 Off-Target Effects
5.2 Delivery Systems
5.3 Emerging Technologies
Thesis Abstract
RNA interference (RNAi) is a powerful cellular mechanism that regulates gene expression by silencing specific target genes. This project aims to investigate the underlying mechanisms of RNAi and its applications in gene regulation. The project will involve studying the biogenesis of small interfering RNAs (siRNAs) and microRNAs (miRNAs), as well as their roles in post-transcriptional gene silencing. Additionally, the project will explore the various techniques used to manipulate RNAi pathways and their potential therapeutic applications. By gaining a deeper understanding of RNAi mechanisms, this project aims to contribute to the development of novel RNA-based therapies.
Thesis Overview
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