1. Introduction
1.1 Background
1.2 Significance of the Study
2. Transcriptional Regulation
2.1 Transcription Factors and DNA Binding
2.2 Chromatin Remodeling Complexes
2.3 Epigenetic Modifications and Gene Expression
3. RNA Processing and Regulation
3.1 Splicing and Alternative Splicing
3.2 RNA Editing and Modification
3.3 RNA Stability and Degradation
4. Translation and Post-Translational Modifications
4.1 Ribosome Function and Protein Synthesis
4.2 Post-Translational Modifications and Protein Activity
4.3 Regulation of Translation Initiation
5. Experimental Methods
5.1 Cell Culture and Transfection
5.2 Chromatin Immunoprecipitation Assays
5.3 RNA Sequencing and Analysis
5.4 Protein Expression and Purification
Gene expression regulation plays a fundamental role in cellular processes and is tightly controlled by various biochemical mechanisms. This project aims to analyze the role of biochemistry in gene expression regulation by investigating the molecular processes involved in transcription, RNA processing, and translation. The project will focus on understanding the biochemical factors that influence gene expression, such as transcription factors, chromatin remodeling complexes, and post-transcriptional modifications. By elucidating the biochemistry of gene expression regulation, this research will contribute to our understanding of cellular function and provide insights into the development of therapeutic interventions
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