1. Introduction
  1.1 Background and significance
  1.2 Objectives of the study
2. Protein Misfolding and Aggregation
  2.1 Definition and causes of protein misfolding
  2.2 Consequences of protein misfolding
  2.3 Factors influencing protein aggregation
3. Chaperone Proteins and Protein Folding
  3.1 Role of chaperone proteins in protein folding
  3.2 Mechanisms of chaperone-assisted protein folding
  3.3 Implications for protein folding diseases
4. Formation of Toxic Protein Aggregates
  4.1 Types of protein aggregates
  4.2 Mechanisms of aggregate formation
  4.3 Relationship between protein aggregates and disease pathology
5. Experimental Methods
  5.1 Techniques for studying protein misfolding and aggregation
  5.2 Animal and cellular models of protein folding diseases
  5.3 Biochemical assays for assessing protein aggregation