1. Introduction
  1.1 Background
  1.2 Objectives
2. Biochemistry Techniques in Biotechnology
  2.1 DNA Manipulation
      2.1.1 Recombinant DNA Technology
      2.1.2 Polymerase Chain Reaction (PCR)
  2.2 Protein Expression Systems
      2.2.1 Prokaryotic Expression Systems
      2.2.2 Eukaryotic Expression Systems
  2.3 Protein Purification Techniques
      2.3.1 Chromatography
      2.3.2 Electrophoresis
3. Biochemical Processes in Biotechnological Applications
  3.1 Recombinant Protein Production
  3.2 Genetic Engineering
  3.3 Biopharmaceutical Development
4. Impact of Biochemistry on Product Yield, Quality, and Safety
  4.1 Yield Optimization Strategies
  4.2 Quality Control and Assurance
  4.3 Safety Considerations
5. Applications of Biochemistry in Biotechnology
  5.1 Industrial Enzyme Production
  5.2 Bioremediation
  5.3 Agricultural Biotechnology