1. Introduction
  1.1 Background
  1.2 Objectives
2. Protein Purification Techniques
  2.1 Chromatography
      2.1.1 Principles
      2.1.2 Types of Chromatography
  2.2 Electrophoresis
      2.2.1 Principles
      2.2.2 Types of Electrophoresis
  2.3 Affinity Purification
      2.3.1 Principles
      2.3.2 Types of Affinity Purification
3. Advantages and Limitations of Protein Purification Techniques
  3.1 Chromatography
  3.2 Electrophoresis
  3.3 Affinity Purification
4. Applications of Protein Purification Techniques
  4.1 Structural Studies
  4.2 Functional Analysis
  4.3 Protein-Protein Interactions
5. Optimization of Protein Purification Protocols
  5.1 Sample Preparation
  5.2 Buffer Optimization
  5.3 Column Selection