Effect of different carbon sources on the growth of antimicrobial producing bacillus species in citrillus vugaris. | Blazingprojects Postgraduate Thesis
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Effect of different carbon sources on the growth of antimicrobial producing bacillus species in citrillus vugaris.

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Carbon Sources
  • 2.2Growth Factors for Bacillus Species
  • 2.3Importance of Antimicrobial Production
  • 2.4Influence of Carbon Sources on Microbial Growth
  • 2.5Previous Studies on Carbon Source Effects
  • 2.6Role of Citrullus Vulgaris in the Study
  • 2.7Impact of Different Carbon Sources on Bacillus Growth
  • 2.8Relationship Between Carbon Sources and Antimicrobial Activity
  • 2.9Comparison of Various Carbon Sources
  • 2.10Future Trends in Carbon Source Research

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Methodology Overview
  • 3.2Research Design and Approach
  • 3.3Sampling Techniques and Participants
  • 3.4Data Collection Methods
  • 3.5Experimental Setup
  • 3.6Data Analysis Procedures
  • 3.7Quality Control Measures
  • 3.8Ethical Considerations

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Overview of Findings
  • 4.2Analysis of Growth Patterns
  • 4.3Comparison of Antimicrobial Production
  • 4.4Effects of Different Carbon Sources
  • 4.5Statistical Analysis Results
  • 4.6Discussion on Bacillus Growth Responses
  • 4.7Interpretation of Antimicrobial Activity Results
  • 4.8Implications of Findings

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Conclusion and Summary
  • 5.2Recap of Study Objectives
  • 5.3Key Findings Overview
  • 5.4Contributions to Existing Knowledge
  • 5.5Practical Applications and Recommendations
  • 5.6Limitations and Future Research Directions
  • 5.7Final Thoughts

Thesis Abstract

Effect of different carbon sources on the growth of antimicrobial producing Bacillus species in ogiri-egusi sold in Ogbeta, Orie, Artisian, Kenyatta, Enugu State were evaluated using standard microbiological and analytical methods. 12 bacteria were isolated and identified such as Bacillus subtilis,(33.33), Bacillus pumulis (41.66), Bacillus licheniformis (25) were identified based on their colony biochemical characteristics.The isolates of the highest occurrences is bacillus pumilus. Agar well diffusion method was used to determine the antimicrobial activity against two pathogenic organisms, Escherichia coli, Staphylococcus aureus. Bacillus subtilis and Bacillus pumulis, gave a zone of inhibition Escherichia coli 0.6mm and 0.8mm after 24 hours of incubation also carbon sources of 5% and 10% sucrose, Glucose and soluble starch were used to check their different carbon sources on the isolates using the spectrophotometer.5% had the highest optimum growth of 1.400 for glucose 1.545 for sucrose,2.376 for soluble starch which can be added in maximum quantity to a medium to produce antibiotics.

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