Assessment of genetic (via protein electrophoresis) and nutritional variabilities among ten accessions of sphenostylis stenocarpa (hocht. ex. a. rich) harms
Table Of Contents
Chapter ONE
INTRODUCTION
- 1.1Introduction
- 1.2Background of Study
- 1.3Problem Statement
- 1.4Objective of Study
- 1.5Limitation of Study
- 1.6Scope of Study
- 1.7Significance of Study
- 1.8Structure of the Research
- 1.9Definition of Terms
Chapter TWO
LITERATURE REVIEW
- 2.1Overview of Genetic Variability
- 2.2Protein Electrophoresis Technique
- 2.3Nutritional Variability in Plants
- 2.4Review of Literature on Sphenostylis stenocarpa
- 2.5Previous Research on Accessions of Sphenostylis stenocarpa
- 2.6Genetic and Nutritional Variability Studies in Similar Crops
- 2.7Impact of Genetic Variability on Plant Traits
- 2.8Relationship between Nutritional Variability and Plant Health
- 2.9Importance of Studying Genetic and Nutritional Variabilities
- 2.10Future Research Directions
Chapter THREE
RESEARCH METHODOLOGY
- 3.1Research Design and Rationale
- 3.2Selection of Accessions for Study
- 3.3Data Collection Methods
- 3.4Genetic Analysis Techniques
- 3.5Nutritional Analysis Procedures
- 3.6Statistical Analysis Plan
- 3.7Ethical Considerations in Research
- 3.8Validation of Results
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- 4.1Analysis of Genetic Variabilities
- 4.2Comparison of Protein Electrophoresis Patterns
- 4.3Assessment of Nutritional Variabilities
- 4.4Correlation between Genetic and Nutritional Traits
- 4.5Factors Influencing Variabilities among Accessions
- 4.6Interpretation of Findings
- 4.7Implications for Crop Improvement
- 4.8Recommendations for Future Studies
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- 5.1Summary of Findings
- 5.2Conclusion
- 5.3Contributions to the Field
- 5.4Practical Applications of Research
- 5.5Limitations and Future Research Directions
Thesis Abstract
The genetic variability among ten (10) accessions of Sphenostylis stenocarpa from three states and National Centre for Genetic Resources and Biotechnology Moor Plantation, Ibadan, Nigeria were assessed using sodium dodecyl polyacrylamide gel electrophoresis (SDS – PAGE). There were variations in the banding patterns of the accessions and polymorphism was also observed. Twelve polypeptide bands were obtained ranging from 9KDA to 219KDa. The Unweighted Pair Group Method with Mean Algorithm (UPGMA) dendrogram grouped the ten accessions into two clusters and six groups. Some accessions appeared in the same group, while others were observed to be in different groups. The proximate and nutritional analysis also detected variations. A strong and positive correlation was observed among some of the attributes studied. The principal component analysis also showed some variation among the accessions. The highest variability was observed in the ash content which shows that ash content could be a major indicator for nutritional variability in these 10 accessions followed by the Vitamin B2 in the second component.
Thesis Overview
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