Isolation of microorganism associated with deterioration of bananas fruits
Table Of Contents
Chapter ONE
INTRODUCTION
- 1.1Introduction
- 1.2Background of study
- 1.3Problem Statement
- 1.4Objective of study
- 1.5Limitation of study
- 1.6Scope of study
- 1.7Significance of study
- 1.8Structure of the research
- 1.9Definition of terms
Chapter TWO
LITERATURE REVIEW
- 2.1Overview of Microorganisms
- 2.2Importance of Microorganisms in Agriculture
- 2.3Role of Microorganisms in Fruit Deterioration
- 2.4Previous Studies on Microorganisms in Fruit Deterioration
- 2.5Factors Affecting Microbial Growth on Fruits
- 2.6Methods for Isolation and Identification of Microorganisms
- 2.7Microbial Control Strategies in Agriculture
- 2.8Impact of Microorganisms on Banana Fruit Quality
- 2.9Relationship Between Microorganisms and Banana Deterioration
- 2.10Future Research Directions in Microbial Studies
Chapter THREE
RESEARCH METHODOLOGY
- 3.1Research Design
- 3.2Sampling Techniques
- 3.3Data Collection Methods
- 3.4Data Analysis Procedures
- 3.5Experimental Setup
- 3.6Statistical Tools for Analysis
- 3.7Ethical Considerations
- 3.8Validation of Results
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- 4.1Overview of Research Findings
- 4.2Microbial Species Identified in Banana Deterioration
- 4.3Factors Influencing Microbial Growth on Bananas
- 4.4Comparison of Microbial Communities on Different Banana Varieties
- 4.5Effects of Environmental Conditions on Microbial Activity
- 4.6Relationship Between Microbial Load and Fruit Quality
- 4.7Control Measures for Microbial Growth on Bananas
- 4.8Implications of Findings on Banana Industry
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- 5.1Conclusion and Summary
- 5.2Recap of Research Objectives
- 5.3Key Findings and Implications
- 5.4Recommendations for Future Research
- 5.5Conclusion Remarks
Thesis Abstract
Abstract
Bananas are one of the most widely consumed fruits globally, but they are susceptible to various microbial infections leading to rapid deterioration during postharvest handling and storage. Understanding the specific microorganisms involved in the deterioration process is crucial for developing effective control measures to prolong the shelf life of bananas. In this study, we aimed to isolate and identify the microorganisms associated with the deterioration of banana fruits. Banana samples showing signs of deterioration, such as discoloration, mold growth, and softening, were collected from local markets and storage facilities. Microbiological analysis was carried out by isolating microorganisms from the surface and inner tissues of the banana samples. The isolated microorganisms were then cultured on specific media to obtain pure cultures for further characterization. The results revealed a diverse array of microorganisms associated with banana deterioration, including bacteria, fungi, and yeasts. Bacterial isolates were identified using biochemical tests and molecular techniques like PCR and sequencing of the 16S rRNA gene. The most common bacterial species identified belonged to the genera Pseudomonas, Bacillus, and Enterobacter. Fungal isolates were characterized based on their macroscopic and microscopic features, as well as molecular analysis using internal transcribed spacer (ITS) sequencing. The predominant fungi isolated included species of Penicillium, Aspergillus, and Fusarium. Yeasts were also isolated and identified using biochemical tests and sequencing of the D1/D2 region of the 26S rRNA gene, with Candida and Saccharomyces being the most prevalent genera. Further investigations were conducted to assess the pathogenicity of the isolated microorganisms on banana fruits through inoculation studies. Pathogenicity tests revealed that certain bacterial and fungal isolates were able to cause decay symptoms similar to those observed in naturally deteriorated bananas. Overall, this study provides valuable insights into the microorganisms associated with banana fruit deterioration and highlights the importance of implementing appropriate control strategies to minimize postharvest losses. By understanding the specific microbial species involved in banana deterioration, targeted interventions can be developed to extend the shelf life of bananas and ensure the delivery of high-quality produce to consumers.
Thesis Overview
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</p><p>INTRODUCTION</p><p>Banana is a long curved fruit which grows in clusters and has soft pulpy flesh and yellow skin when ripe.There are about 1000 varieties of bananas in the world. Banana is an edible fruit botanically a berry, produced by several kinds of large herbaceous flowering plants in the genus Musa. In some countries bananas used for cooking may be called plantains. The fruit is variable in size, colour and firmness, but is usually elongated and curved, with soft flesh rich starch covered with a rind which may be green, yellow, red, purple, or brown when ripe, (Mwan et al., 2007).</p><p>In the developing world, banana is a major staple crop of considerable importance. Most producers and consumer of banana determines the quality of the banana by the colour of the peels. The colour of the fruit could serve as an indication of the state of deterioration, disease and/or confirmation (Crane et al., 2005).</p><p>1.1 Background of the study</p><p>In 2013, Canadians spent 875 billion on food, the largest share was spent on fruit production.</p><p>The global fruit processing industry has experienced consistent increased demand over the last five years, for processed fruit and fruit products and consumer spending has increased. Demand has grown particularly faster in developing economies as industrial growth has translated into greater urbanization, higher percental income and expansion in the size of the middle class has grown it has demanded larger quantities of higher quality more diverse food. Increase consumption of fruits can be attributed to more household becoming health centered (CSA, 2004).</p><p>Microorganisms, natural disaster such as earthquakes etc. has cause a substantial damage to fruit production and has naturally affected the Global fruit production.</p><p>The quantity of bananas product in Nigeria has not been estimated. However it is known to be produced in varying amount within the following states: Abia, Awkaibom, Anambra, Benue, Cross River, Delta, Enugu, Kaduna and Ondo (FOS, 2015)</p><p>Because of the massive production of banana fruit and poor storage and handling process, leads to spillage of banana fruit during the post-harvest period, (CSA, 2004).</p><p>1.2 Statement of Problem</p><p>Because of the world wide massive production of banana and as result of poor storage facilities this leads to large waste of banana due to spoilage</p><p>Microbial associated spoilage mostly is due to contamination from external sources during post harvest practices as a result of transport to the market or during storage of the fruit.During the ripening process, there are gradual series of changes in the colour of the banana from green to yellow. One should be able to distinguish between spoilage and ripening of banana.</p><p>Most banana affected with microorganisms (fungi or/and bacteria) usually get spoilt most at times at the middle of the ripening process or when the banana has become ripe.Banana fruit can be contaminated by microorganisms through skin penetration, natural opening or mechanical damage. There are two main microbes (Fungi and Bacteria) involve in spoilage of banana, which reduce the quality of the banana fruit causing the fruit to be consideration and unmarketable. The storage life of banana fruits can be improved using low temperature , 90% humility, removal of ethylene storing in 5% CO2 and 3% oxygen at 280c, use of chemicals and irradiation, use of packaging materials and fruit processing.</p><p>1.3 Justification</p><p>There is highly consumption of banana in Nigeria also high loss due to spoilage.</p><p>The study centre on microorganisms associated with spoilage in fresh and spoilt banana. Thus this study will provide a data base on microbial spoilage of banana. Microorganism</p><p>1.4 Aim of the study</p><p>To ISOLATE MICROORGANISM ASSOCIATED WITH DETERIORATION OF BANANAS FRUITS and to assess common ways that have used to improved the shelf life of the fruit sold in Makurdi Benue state.</p><p>1.5 Specific objective of the study</p><p>To isolate and identify micro organism associated with spoilage fresh Banana fruits in Makurdi.To isolate and identify micro organism associated with spoilt banana fruits sold in Makurd.The distribution of spoilage micro organisms among markets in Makurdi. MicroorganismTo determine the physio chemical parameters of banana pulp.</p>
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