Microbial profile of suya meat | Blazingprojects Postgraduate Thesis
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Microbial profile of suya meat

 

Table Of Contents


  •  LIST OF TABLESTABLE 1: Total viable and coliform counts ()TABLE 2: Characterization /identification of IsolateTABLE 3: Frequency of occurrence of isolates                                 TABLE OF CONTENTS Certification                                                                                                     iDedication                                                                                                     iiAcknowledgement                                                                                        iiiAbstractTableofcontents                                                                                                    ivList of Tables                                                                                                vCHAPTER ONE1.0 Introduction                                                                                            
  • 11.1Objectives                                                                                                 4CHAPTER TWO2.0 Literature review                                                                                     52.1Suya meat                                                                                                
  • 52.2Preparation of suya                                                                                      52.3Preparation of meat2.4 Meat spoilage2.5 Factors that affect the growth of microorganisms in meat2.
  • 5.1Temperature2.
  • 5.2pH2.5.
  • 3.Water availability2.
  • 5.4NutrientsCHAPTER THREE3.0 Materials and methods3.1 List of reagents3.2 List of glassware3.3 Preparation of media3.4 Sample collection3.5 Preparations of samples3.6 Determination of total viable counts3.7 Characterization and Identification of Isolates3.
  • 7.1Gram reaction3.
  • 7.2Motility test3.
  • 7.3Catalase test3.
  • 7.4Coagulase test3.
  • 7.5Oxidase test3.
  • 7.6Urease test3.
  • 7.7Citrate test3.
  • 7.8Vogues Proskauer test3.
  • 7.9Indole test3.
  • 7.10Carbohydrate fermentation test3.
  • 7.11Methyl red testCHAPTER FOUR4.0 ResultsCHAPTER FIVE5.1 Discussion5.2 Summary and Conclusion5.3 RecommendationsReferencesAppendixLIST OF TABLESTABLE 1: Total viable and coliform counts ()TABLE 2: Characterization /identification of IsolateTABLE 3: Frequency of occurrence of isolates

Thesis Abstract

Ten (10) samples of suya meat in Enugu were collected randomly and analyzed microbiologically and the isolates were identified as Staphylococcus aureus (35%)Escherichia coli (15%) Streptococcus species (15%) Pseudomanas (35%) . The most frequently isolated organism was Staphylococcus and Pseudomonas. The total viable bacterial counts ranged from 1.9x 3.8x cfu/g whereas, total coliform count ranged 1.1x-3.0x cfu/g on MacConkey and Nutrient agar respectively. The result revealed that the hygienic condition of the meat have fallen below acceptable standard for human comsumption.

Thesis Overview

<p> </p><p><strong>1.0</strong>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; <strong>&nbsp;INTRODUCTION</strong></p><p><strong>1.1</strong><strong>&nbsp; &nbsp; &nbsp; BACKGRONUD OF THE STUDY</strong></p><p>Meat is the flesh of animals which serves as food; it is obtained from sheep, cattle, goat and swine (Hamman, 1997). Meat is a major source of protein and have valuable qualities of vitamins for most people in many parts of the world, thus they are essential for the growth, repair and maintenance of body cells which is necessary for our everyday activities.</p><p>Meat could be traced back to human history, then when primitive men use raw flesh of dead animals, but as man developed, he domesticated as well as wild animals. Beef have been the major supply of meat in Nigeria as a result of extensive and semi-intensive cattle production system in Nigeria by Fulani and Hausa people of the northern Nigeria. (Umoh, 2004).</p><p>Suya meat is a boneless lean meat of mutton, beef, goat or chicken meat staked on sticks, coated with its sauces, oiled and then roasted over wood using a fire from charcoal. It is a popular, traditionally processed meat product that is served hot and sold along streets, at clubs, picnics centers, and restaurants and within institutions. Suya meat is one of the intermediate moisture products that are easy to prepare and highly relish which is a mass consuming fast food and its preparation and sales are usually not done with strict hygiene condition because they are still done locally.</p><p>Due to the chemical composition and characteristic, meat are highly perishable food which provides excellent source of growth of many hazardous microorganisms that can cause infection in human and also lead to meat spoilage and economic loss. The most important bacteria meat spoilage is caused by lactic acid bacteria which is physiologically related group of fastidious and ubiquitous gram-positive organisms, these includes many species such as <em>Lactobacillus, Leuconostoc, Pediococcus and Streptococcus.</em></p><p>Since meat has a high nutritive value, microorganisms could easily grow on it. The possible sources of contamination are through slaughtering of sick animals, washing the meat with dirty water, handling by butchers, contamination by flies, processing close to sewage or refuse dumps environment, spices, transportation and use of contaminated equipment such as knife and other utensils. (Igyor and <em>Uma, 2005).</em></p><p>The slaughtering process affords extensive contamination of sterile tissue with gram-negative enteric bacteria from animal intestine including <em>Salmonella specie</em>and <em>Escherichia coli</em>&nbsp;as well as contaminant such as gram-positive <em>Lactic cocci</em>associated with humans, animals and the environment. <em>Enterococci </em>and <em>Clostridia</em>have been isolated from lymph node of red meat animals (Lawries, 2000, Alexander<em>et. al</em>. 1998).</p><p>Microorganisms grow on meat causing visual, textural and organoleptic changes when they release metabolite (Jackson <em>et. al</em>. 2001). The smoke produced as a number of effects including preservative effect resulting from the deposition of organic compounds all presents in the smoked product (Suya meat). (Dineen<em>et.al</em>.1999). A preservative effect is also induced by the surface drying that occurs to the extent of 30% total weight loss in hot smoked product. Antioxidant effect is produced by the phenolic deposite unto the product.</p><p>The microbial load in meat and meat product increases as long as growth conditions are favorable. The factor influencing microbial growth includes acidity pH, temperature, water activity, gaseous requirement, nutrient and competition of microbes for the nutrient. Controlling these factors implies maintaining long shelf life of meat and meat product but proper preservation of meat could be achieved by the combination of two or more preservation method which includes drying, salting and high temperature (Nester <em>e, al</em>&nbsp;2001).</p><p><strong>1.2 &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; AIMS AND OBJECTIVES</strong></p><p>This work is aimed at determining the microbial quality of suya meat sold in Enugu and has the following objectives:</p><p>1. &nbsp;To isolate, characterize and identify microbial species associated with &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Suya meat.</p><p>2. To establish the public health implication of consumption of Suya meat.</p><p>3. To offer useful information where necessary to the consuming public.</p> <br><p></p>

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