EVALUATION OF THE PERFORMANCE OF SECCHAROMYCES CEREVISIAE ISOLATE FROM PALM-WINE IN VINEGAR PRODUCTION FROM OR ANGE JUICE. | Blazingprojects Postgraduate Thesis
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EVALUATION OF THE PERFORMANCE OF SECCHAROMYCES CEREVISIAE ISOLATE FROM PALM-WINE IN VINEGAR PRODUCTION FROM OR ANGE JUICE.

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Vinegar Production
  • 2.2History of Saccharomyces cerevisiae in Fermentation
  • 2.3Role of Microorganisms in Vinegar Production
  • 2.4Factors Affecting Vinegar Production
  • 2.5Types of Vinegar
  • 2.6Biotechnological Applications in Vinegar Production
  • 2.7Quality Control in Vinegar Production
  • 2.8Fermentation Process in Vinegar Production
  • 2.9Benefits of Vinegar Consumption
  • 2.10Innovations in Vinegar Production

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Methodology Overview
  • 3.2Selection of Saccharomyces cerevisiae Isolate
  • 3.3Experimental Design for Vinegar Production
  • 3.4Data Collection Methods
  • 3.5Statistical Analysis Techniques
  • 3.6Sampling Techniques
  • 3.7Laboratory Procedures
  • 3.8Research Ethics and Compliance

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Analysis of Saccharomyces cerevisiae Performance
  • 4.2Comparison of Vinegar Production Methods
  • 4.3Evaluation of Fermentation Parameters
  • 4.4Chemical Composition Analysis of Vinegar
  • 4.5Microbial Analysis of Vinegar Samples
  • 4.6Sensory Evaluation of Vinegar Products
  • 4.7Discussion on Process Optimization
  • 4.8Interpretation of Results

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Conclusion and Summary
  • 5.2Summary of Findings
  • 5.3Implications of Research
  • 5.4Recommendations for Future Studies
  • 5.5Contribution to Knowledge

Thesis Abstract

This project work aims at understanding the role of yeast in the vinegar production. This involves the breaking down of glucose molecule in the orange must into ethanol and carbon dioxide and water. The strains of the yeast used was sacharomyces cerevisiae isolated from palm-wine. The procedure and processes of isolating it which involves the palm-wine on the nutrient media after contrifugating the palm-wine and the supernatant was discarded. The isolated pure yeast cell was cultured with a selective yeast extract agar (YEA) to produce enough inoculum for the fermentation of the orange must. The yeast was inoculated into the orange must and the action of the yeast (seccharomyces cerevisiae) was careful examined to understand and observe the performance of the yeast in the production of vinegar from orange must. The activities which was examined daily by careful observation of the plated culture to evaluate the rate of changes from first day till the fifth day of the fermentation process.

Also, gram stain reaction was carried out to identify the possible contaminant of the inoculated culture. The result was tabulated and described based on the various test carried out and the colonies of the possible contamination was desecribed.

Thesis Overview

<p><br>INTRODUCTION<br><br>Vinegar is an alcoholic liquid that has been allowed to allowed to sour. It is primarily use to flavour and preserve foods and ingredient in salad dressing and marinades. Vinegar is also use in a cleaning agent. The word is from the French vin (wine) an aigre (sour) costman et al, 2005).<br><br>Vinegar is made from the oxidation of ethanol in wine, cider beer, fermented fruit juice or nearly any other liquid containing alcohol. It is generally known to be made from a variety of diluted alcoholic products, the most common being wine, beer and rice. Commercially, vinegar is produced either by fast or slow fermentation process. Slow methods are generally used with traditional vinegars and fermentation proceeds slowly over the course of weeks or months. The longer fermentation proceeds slowly over for the accumulation of nontoxic slime composed of acetic acid bacterial and soluble cellulose, known as the mother of vinegar. Fast methods adds mother of vinegar ie bacterial culture to the source liquid and then add air using a venture pump system or a turbine to promote oxygenation to give the fastest fermentation. In fast production processes, vinegar may be produce in a period ranging from 20 hour to three days (Johnston et all, 2004).<br></p>

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