Hydrolysis of cassava starch by amylases from maize

Thesis Abstract

Abstract
The hydrolysis of cassava starch using amylases derived from maize is a significant area of research due to the potential applications in various industries. This study aimed to investigate the efficiency of maize-derived amylases in breaking down cassava starch into simpler sugars. The process involved optimizing the reaction conditions such as temperature, pH, enzyme concentration, and reaction time to maximize the hydrolysis efficiency. The results indicated that the amylases from maize effectively hydrolyzed cassava starch, leading to the production of glucose and other maltose sugars. The optimal conditions for the hydrolysis were found to be a temperature of 50°C, pH 5.5, enzyme concentration of 2%, and a reaction time of 60 minutes. Under these conditions, the amylases showed a high catalytic activity, resulting in a significant breakdown of cassava starch. Furthermore, the kinetic parameters of the enzymatic hydrolysis were determined, showing the Vmax and Km values of the maize-derived amylases for cassava starch. The study also investigated the effects of inhibitors and activators on the enzymatic activity, providing insights into the factors that can influence the efficiency of the hydrolysis process. Overall, the findings of this research contribute to the understanding of the hydrolysis of cassava starch by amylases from maize and provide valuable information for potential industrial applications. The optimized conditions and kinetic parameters obtained in this study can serve as a basis for further research in the development of efficient enzymatic processes for starch hydrolysis. The use of maize-derived amylases for cassava starch hydrolysis can have significant implications for industries such as food, pharmaceuticals, and biofuel production, where the conversion of starch into sugars is essential. In conclusion, this study demonstrates the potential of maize-derived amylases for the hydrolysis of cassava starch, highlighting their effectiveness in breaking down complex carbohydrates into simpler sugars. The optimized conditions and kinetic parameters obtained in this research pave the way for future studies aiming to enhance the efficiency of enzymatic processes for starch hydrolysis, with broader implications for various industrial applications.