Microorganism associated with spoilage of tomatoes (canned tomatoes) | Blazingprojects Postgraduate Thesis
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Microorganism associated with spoilage of tomatoes (canned tomatoes)

 

Table Of Contents


  • Chapter one1.0        IntroductionChapter Two2.0        Literature review2.1   History of canned tomato2.2   Types of canned Tomatoes2.3   Nutrition facts of canned tomatoes2.4   Common additions of canned tomato2.5   causes of canned tomatoes spoilage2.6   Control/preservation of canned tomatoChapter Three3.0        Materials and Methods3.1   Place of experimental work3.2   materials used3.
  • 2.1Reagent used3.3   Collection of sample3.4   Medium preparation3..
  • 4.1      Preparation of nutrient agar3.
  • 4.2Preparation of potato dextrose agar3.5   Sample preparation3.6   Microbiological Analysis of the Sample3.
  • 6.1Determination of analysis load (bacterial and Fungi Load)
  • 3.7  Preparation of pure cultures pf Isolate (bacteria and fungi)
  • 3.7  Identification of bacteria isolates3.
  • 7.2Colonial or cultural characteristics3.8   Gram Staining3.
  • 8.1Indole test3.
  • 8.2Motility Test using stab cultures techniques3.
  • 8.3Methyl Red (Mr ) Test3.
  • 8.4Volies –proskader (VIPs) test3.
  • 8.5Identification of fungi3.9   Microscopic Examination3.10 biochemical ReactionsChapter Four4.0        Microbial Load or bacteria Associated with canned Tomato spoilage4.1   Discussion4.2   Conclusion4.3   Recommendation          References

Thesis Abstract

The micro-organism, associated with spoilage of tomatoes shows that tomato contain large amount of water which makes them more susceptible to spoilage by the action of various micro-organism. Out of the (3) three sources of samples used in the assessment revealed that Bacillus species identified in the spoiled sample are the major causes of canned spoilage while the fungi isolates are peniucillum species etc which revealed by the assessment are also the cause of tomato spoilage. In this assessment carried out, it was found out that bacterial species were the source of spoilage rather than bacteria because it dominated all the plates.

Thesis Overview

<p> </p><p><strong>INTRODUCTION</strong></p><p>Canned tomatoes are tomatoes usually peeled that are sealed into a canned after having been processed by heat.</p><p>This product are considered important world wide (Robinso, et al, 1994). In Nigeria tomato paste is the most important tomato product because of its wide spread use for preparation of various food/menus. It contain on the average about 6.4% total solids, of which 3.5% is invert sugar, 0.5% citric acid,0.6% ash, 0.9% protein, 0.53% crude fibre and about 0.05% fat. When spoil as a result of the life processes of bacteria, yeast and molds, the sugars are rapidly used up being changes into acetic acid, lactic acid, alcohol and carbon dioxide, the amount of these substances depending on the types of organism which are most active in the particular sample in question.</p><p>Until recent years, nearly all the tomato paste consumed in Nigeria was important from European countries with Italy being the leading country. However, today, limited brands are produced locally. Although both the imported and the local brands are available on the market, consumers prefer the former in spite of its relatively higher cost.</p><p>In general, adequate heat processing is given to tomato paste to achieve commercial sterility (speck, 1984), but subsequent abusive post-process handling/storage may lead to undesirable microbiological changes (Anon, 1980). It is public knowledge that can of tomato paste often show external evidence of spoilage under tropical retail conditions. In addition, and interestingly, these d effective products are sold (especially to the less informed) at the same cost as the normal (non-defective- product.</p><p>The structural &nbsp; systematic approach for the control of food safety “Hazard Analytsis and critical control point-HACCP” identifies, evaluated and controls hazards that are significant for food safety. The control parameters include various factors such as time of harvesting, temperature and moisture during storage, selection of agricultural products prior to processing, decontamination conditions, addition of chemicals at final product storage ((Sango, 1995).</p> <br><p></p>

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