Evaluation of elephant grass (pennisetum purpureum) as substrate for bioethanol production using co-cultures of aspergillus niger and saccharomyces cerevisiae | Blazingprojects Postgraduate Thesis
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Evaluation of elephant grass (pennisetum purpureum) as substrate for bioethanol production using co-cultures of aspergillus niger and saccharomyces cerevisiae

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Bioethanol Production
  • 2.2Properties of Elephant Grass (Pennisetum purpureum)
  • 2.3Aspergillus niger in Bioethanol Production
  • 2.4Saccharomyces cerevisiae in Bioethanol Production
  • 2.5Co-cultures of Aspergillus niger and Saccharomyces cerevisiae
  • 2.6Biochemical Pathways in Bioethanol Production
  • 2.7Factors Affecting Bioethanol Yield
  • 2.8Comparative Studies on Substrates for Bioethanol Production
  • 2.9Advancements in Bioethanol Production Technologies
  • 2.10Environmental Impact of Bioethanol Production

Chapter THREE

SYSTEM DESIGN AND IMPLEMENTATION

  • 3.1Research Design and Methodology
  • 3.2Selection of Elephant Grass as Substrate
  • 3.3Cultivation and Harvesting of Elephant Grass
  • 3.4Preparation of Co-cultures of Aspergillus niger and Saccharomyces cerevisiae
  • 3.5Fermentation Process for Bioethanol Production
  • 3.6Optimization Techniques for Bioethanol Yield
  • 3.7Analytical Methods for Bioethanol Quantification
  • 3.8Statistical Analysis of Data

Chapter FOUR

SYSTEM TESTING AND EVALUATION

  • 4.1Bioethanol Yield from Elephant Grass Substrate
  • 4.2Effect of Co-cultures on Bioethanol Production
  • 4.3Comparative Analysis with Single Culture Fermentation
  • 4.4Optimization of Fermentation Parameters
  • 4.5Economic Viability of Bioethanol Production
  • 4.6Environmental Sustainability Assessment
  • 4.7Challenges and Solutions in Bioethanol Production
  • 4.8Future Prospects and Recommendations

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Findings
  • 5.2Conclusion
  • 5.3Implications for the Industry
  • 5.4Recommendations for Further Research
  • 5.5Closing Remarks

Thesis Abstract

Elephant grass (Pennisetum purpureum) was evaluated for its ethanol production potential using co-cultures of Aspergillus niger and Saccharomyces cerevisiaeisolated from local sources. Proximate and lignocellulose analysis carried out on the plant sample showed that it had crude fibre, lignin, hemicellulose and cellulose contents of 31.5%, 26.78%, 18.76% and 34.16% respectively. Aspergillus niger strains were isolated from soil and bread and were further screened for both qualitative and quantitative cellulase production. Qualitative cellulase assay revealed clear zones around colonies indicative of enzyme activity on solid agar medium containing 0.1% carboxymethyl cellulose (CMC) for all the isolates. Quantitative cellulase assay showed that A. niger isolate AN-15 from soil gave highest cellulase yield of (0.1792 IU/ml/min) and was therefore selected as a co-culture with

S. cerevisiae. Saccharomyces cerevisiae strains were isolated from palm wine and burukutu.Isolate PW-4 was selected for fermentation based on ethanol tolerance tests and assimilation of more sugars compared to other isolates. Fermentation of grass substrate was carried out at different concentrations ranging from 2-10% and highest ethanol yield of 1.68g/100ml was observed at an optimum substrate concentration of 6% though the yield was much less than that obtained from equal concentration of glucose (8.38g/100ml). Optimization of culture parameters for ethanol production showed maximum ethanol yield at pH 5, 35oC and agitation rate of 300 rpm. The results of the research also revealed that ethanol production by S. cerevisiae beyond the fourth day of fermentation is significantly reduced.

 


Thesis Overview

<p> </p><p><strong>1.0</strong>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;<strong>INTRODUCTION</strong></p><div>Ethanol fuel, ethyl alcohol (CH3CH2OH), is the same type of alcohol found in alcoholic beverages. It is an oxygenated fuel with a high octane value like that of petroleum fuels known to run combustion engines at higher compression ratios and thus provides superior performance (Wheals <em>et al</em>., 1999). The blending of ethanol into petroleum-based automobile fuels can significantly decrease petroleum use and decrease the release of greenhouse gas emissions. Furthermore, ethanol can be a safer alternative to the common additive, methyl tertiary butyl ether (MTBE), in gasoline. Methyl tertiary butyl ether is toxic and is a known contaminant in ground water. Thus, ethanol can be a substitute to mitigate the problems associated with the rising energy demands across the world as well as a way to reduce greenhouse gas emission to as high as 85% (Perlack <em>et al</em>., 2005).</div><p>Ethanol may be produced either from petroleum products or from biomass substrate. Today, most of the ethanol produced comes from renewable resources (Bothast and Saha, 1997). Although, most of the ethanol currently produced from renewable resources come from sugarcane and starchy grains, significant efforts are being made to produce ethanol from lignocellulosic biomass (almost 50% of all biomass in the biosphere such as agricultural residues are lignocellulosic biomass). The technological advances in recent years are promising to produce ethanol at low cost from lignocellulosic biomass (Bothast and Saha, 1997).</p><p>Bioethanol production from sugarcane and starch-rich feed stocks such as corn, potato, is considered a first generation process because it has already been developed (Joshi <em>et al</em>., 2011).</p> <br><p></p>

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