Effect of different carbon sources on the growth of antimicrobial producing (bacillus species in citrillus vugaris) | Blazingprojects Postgraduate Thesis
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Effect of different carbon sources on the growth of antimicrobial producing (bacillus species in citrillus vugaris)

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Antimicrobial Producing Bacillus Species
  • 2.2Importance of Carbon Sources in Microbial Growth
  • 2.3Types of Carbon Sources
  • 2.4Influence of Carbon Sources on Microbial Metabolism
  • 2.5Factors Affecting Microbial Growth
  • 2.6Role of Carbon Sources in Antimicrobial Production
  • 2.7Previous Studies on Carbon Sources and Microbial Growth
  • 2.8Relationship Between Carbon Sources and Bacillus Species
  • 2.9Experimental Design in Studying Carbon Sources
  • 2.10Summary of Literature Review

Chapter THREE

SYSTEM DESIGN AND IMPLEMENTATION

  • 3.1Research Methodology Overview
  • 3.2Research Design and Approach
  • 3.3Sampling Techniques
  • 3.4Data Collection Methods
  • 3.5Data Analysis Procedures
  • 3.6Variables and Measurements
  • 3.7Ethical Considerations
  • 3.8Research Limitations and Challenges

Chapter FOUR

SYSTEM TESTING AND EVALUATION

  • 4.1Data Analysis and Interpretation
  • 4.2Growth Patterns of Bacillus Species on Different Carbon Sources
  • 4.3Antimicrobial Production Levels in Relation to Carbon Sources
  • 4.4Comparison of Growth Rates on Various Carbon Sources
  • 4.5Statistical Analysis of Results
  • 4.6Discussion on Findings
  • 4.7Implications of Results
  • 4.8Recommendations for Future Research

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Findings
  • 5.2Conclusions Drawn from the Study
  • 5.3Contributions to Existing Knowledge
  • 5.4Practical Applications of the Research
  • 5.5Recommendations for Further Study
  • 5.6Conclusion and Final Remarks

Thesis Abstract

Effect of different carbon sources on the growth of antimicrobial producing Bacillus species in ogiri-egusi sold in Ogbeta, Orie, Artisian, Kenyatta, Enugu State were evaluated using standard microbiological and analytical methods. 12 bacteria were isolated and identified such as Bacillussubtilis,(33.33), Bacillus pumulis (41.66),

Bacillus licheniformis (25) were identified based on their colonybiochemical characteristics.The isolates of the highest occurrences is bacillus pumilus. Agar well diffusion method was used to determinethe antimicrobial activity against two pathogenic organisms,

Escherichia    coli,     Staphylococcus    aureus.     Bacillus     subtilis     and

Bacillus pumulis, gave a zone of inhibition Escherichia coli 0.6mmand 0.8mm after 24 hours of incubation also carbon sources of 5% and 10% sucrose, Glucose and soluble starch were used to check their different carbon sources on the isolates using the spectrophotometer.5% had the highest optimum growth of 1.400 for glucose 1.545 for sucrose,2.376 for soluble starch which can be added in maximum quantity to a medium to produce antibiotics.


Thesis Overview

<p> </p><p><strong>1.0 &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; </strong><strong>INTRODUCTION</strong></p><p>Traditional &nbsp;fermented &nbsp;condiments &nbsp;(OGIRI-EGUSI) &nbsp;based &nbsp;on</p><p>vegetable proteins are consumed by different ethnic groups in Nigeria, have been the pride of culinary traditions for centuries. It is evident that these products have played a major role in the food habits of communities in the rural regions serving not only as nutritious non-meat proteins substitute but also as condiment and flavoring agents in soup.</p><p>Traditional methods of manufacture should take advantage of biotechnological progress to assure reasonable quality and at the same time assure safety of these products. The requirements for a sustainable biotechnological development of Nigerian condiments are discussed in the scope of the microbiology and biochemical changes of the raw materials. Fermented vegetables, proteins have potential food uses as protein supplements and as functional ingredients in the fabricated food (Achi. 2005).</p> <br><p></p>

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