Comparative study of disinfectant efficiency of ethanol, bleach and phenolics against pseudomonas aeruginosa and staphylococcus aureus
Table Of Contents
Chapter ONE
INTRODUCTION
- 1.1Introduction
- 1.2Background of study
- 1.3Problem Statement
- 1.4Objective of Study
- 1.5Limitation of Study
- 1.6Scope of Study
- 1.7Significance of Study
- 1.8Structure of the Research
- 1.9Definition of Terms
Chapter TWO
LITERATURE REVIEW
- 2.1Overview of Disinfectants
- 2.2Ethanol as a Disinfectant
- 2.3Bleach as a Disinfectant
- 2.4Phenolics as a Disinfectant
- 2.5Mechanisms of Action
- 2.6Efficacy Studies on Pseudomonas aeruginosa
- 2.7Efficacy Studies on Staphylococcus aureus
- 2.8Comparative Studies on Disinfectant Efficiency
- 2.9Factors Affecting Disinfectant Performance
- 2.10Current Trends in Disinfectant Research
Chapter THREE
SYSTEM DESIGN AND IMPLEMENTATION
- 3.1Research Methodology Overview
- 3.2Selection of Disinfectants
- 3.3Culturing of Pseudomonas aeruginosa and Staphylococcus aureus
- 3.4Experimental Design
- 3.5Disinfection Protocols
- 3.6Data Collection Methods
- 3.7Statistical Analysis Techniques
- 3.8Ethical Considerations in Research
Chapter FOUR
SYSTEM TESTING AND EVALUATION
- 4.1Data Analysis and Interpretation
- 4.2Disinfectant Efficacy Results
- 4.3Comparison of Disinfectant Performance
- 4.4Discussion on Pseudomonas aeruginosa Findings
- 4.5Discussion on Staphylococcus aureus Findings
- 4.6Factors Influencing Disinfectant Efficiency
- 4.7Implications of Research Findings
- 4.8Recommendations for Future Studies
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- 5.1Conclusion and Summary
- 5.2Recap of Research Objectives
- 5.3Key Findings Recap
- 5.4Contributions to the Field
- 5.5Practical Applications of Research
- 5.6Limitations of the Study
- 5.7Suggestions for Further Research
- 5.8Final Thoughts
Thesis Abstract
Ethanol, Bleach and Phenolics are three kinds of disinfectants which have been widely used in common laboratories. In this study, a compared experiment on these three disinfectants efficiency was conducted against Staphylococcus aureus and Pseudomonas aeruginosa using agar hole diffusion method. Different concentrations of bleach (1%, 2%, 3%, 4% and 5%) were used on both organisms. Also (50%, 60%, 70%, 85% and 95%) of ethanol as well as (5%, 10%, 20%, 25%, and 30%) Phenolics were used. Diffrences in concentrations tested was because, the original concentrations of the disinfectants differs. After 24 hours of incubation at 370C, the results showed that all the disinfectants inhibited the growth of the test organism in their concentrated forms. The diameter of zone of inhibitions were measured around each well by using a ruler in millimeters, using different concentrations, their efficacies varied. The results showed that 30% Phenolics had the best efficiency against both test organisms and 5% bleach had a better effect on Staphylococcus aureus than Pseudomonas aeruginosa, while ethanol showed least sensitivity. 70% concentration gave the highest effect on Staphylococcus aureus as compared withPseudomonas aeruginosa.
Thesis Overview
<p> </p><p>1.0 INTRODUCTION</p><p> Microorganisms are minute living things that individually are too small to be seen with the unaided eyes (Tortora <em>et al</em>, 2007). Though only a minority of microorganisms are pathogenic (disease producing), practical knowledge of microbes is necessary for medicine and related health sciences. For example hospital workers must be able to protect patients from common microbes that are normally harmless but pose a threat to the sick and injured. Thousands of people died in devastating epidemics; the cause of which was not understood. Entire families died because vaccination and antibiotics were not available to fight infection (Johnson and Case, 1995). This leads to scientific control of microbial growth. This began only about 100 years ago. It was Pasteur’s work on microorganism that led scientists to believe that microbes were a possible cause of diseases and need to be eliminated or destroyed. Some examples off these microbes are; Bacteria, fungi, viruses and protozoa etc (Tortora et al, 2007).</p> <br><p></p>Blazingprojects Mobile App
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