Extraction, characterization and anti microbial screening of white star apple (crysophyllum albidum) seed oil | Blazingprojects Postgraduate Thesis
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Extraction, characterization and anti microbial screening of white star apple (crysophyllum albidum) seed oil

 

Table Of Contents


  •                                                                   Title page                                                                               Declaration                                                                                             Certification                                                                                     Dedication                                                                                               Acknowledgment                                                                                   Table of content                                                                           Abstract                                                                                          

Chapter ONE

INTRODUCTION

  •  
  • 1.1  INTRODUCTION                                                                   LITERATURE REVIEW                                                                
  • 1.2  TYPES OF OIL                                                                     1.
  • 2.1Drying oil                                                                                 1.
  • 2.2Semi-Drying Oil                                                                    NON- DRYING OIL                                                    
  • 1.3  COMPOSITION OF OIL                                                      
  • 1.4  PIGMENT                                                                          VITAMINS                                                                                       ANTIOXIDANTS                                                                            
  • 1.7  BOTANIC DESCRIPTION                                                  
  • 1.8  FUNCTIONAL USES                                                          

Chapter TWO

LITERATURE REVIEW

  •  MATERIALS AND METHODS                                                    
  • 2.0  SAMPLE COLLECTION                                                      
  • 2.1  OIL EXTRACTION                                                              
  • 2.2  DETERMINATION OF SPECIFIC GRAVITY                    
  • 2.3  DETERMINATION OF DENSITY                                      
  • 2.4  DETERMINATION OF FREE FATTY ACID CONTENT                                                                            DETERMINATION OF SAPONIFICATION VALUE          DETERMINATION OF IODINE VALUE                          DETERMINATION OF PEROXIDE VALUE                     ANTI-MICROBIAL ACTIVITY SCREENING                     REAGENTS AND MEDIA                                             PREPARATION OF FUNGAL TEST ORGANISM          PREPARATION OF THE SENSITIVE TEST AGAR                  PREPARATION OF THE NUTRIENT DEXTROSEAGAR                                                                                     2.
  • 8.5THE PUNCHED AGAR DIFFUSION METHOD AS RECOMMENDED BY BRYANT (1972)                             2.
  • 7.6BACTERIAL INOCULATION AND INCUBATION             2.
  • 8.7FUNGAL INOCULATION AND INCUBATION                            READING INHIBITION ZONES OF THE OIL                    

Chapter THREE

RESEARCH METHODOLOGY

  •  TABLE
  • 3.1ORGANOLEPTIC CHARACTERISTICS OF Crysophylum albidum SEED OIL                                                
  • 3.2  CHARACTERIZATION OF THE SAMPLE                         TABLE
  • 3.3RESULT OF ANTI-BACTERIAL ACTIVITY OF C. albidum OIL ON TWO GRAM POSITIVE BACTERIA                 TABLE
  • 3.4RESULT OF ANTI-BACTERIAL ACTIVITY OF C. albidum OIL ON TWO GRAM NEGATIVE BACTERIA               TABLE
  • 3.5RESULT OF ANTI-FUNGAL ACTIVITY OF C. albidum OIL ON TWO TEST FUNGI                                                          
  • 3.6  RESULT OF MINIMUM INHIBITORY CONCENTRATION (MIC) OF C. albidum OIL ON SIX TEST ORGANISM                

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  •  
  • 4.0  DISCUSSION                                                                    
  • 4.1  CONCLUSION                                                                   RECOMMENDATION                                                          REFERENCES                                                                               APPENDIX I                                                                                    

Thesis Abstract

Crysophylum albidum oil was extracted from its seed. The percentage yield was 2.56%. The characterization of the oil showed showed that the refractive index is 1.487, peroxide value is 45.4mg/kg, iodine value is 50.76g, saponification value is 105.188, free fatty acids 47.46 and acid value is 94.92. The Punched Ager Diffusion Method was used to assay for the antimicrobial and anti fungal properties of the oil in the test isolate. The antimicrobial and anti fungal activity showed some inhibitory effects against test organisms; Staphylococcus aureus, E. coli, B. subfilis, C. albican and A. flavons, but non for S. pyogens. The minimum inhibitory concentration of these test organisms are as follows; Staphylococcus aureus 0.16, E. coli 0.06, B. subfilis 0.14, C. albican 2.50 and A. flavons  0.40. The pharmacological screening confirmed the medical value of this plant oil and it established a good support for the sample in herbal medicine and as a base for the development of new drugs and phytomedicine.

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