Phytochemical analysis of cleome viscosa.
Table Of Contents
Chapter ONE
INTRODUCTION
- 1.1Introduction
- 1.2Background of study
- 1.3Problem Statement
- 1.4Objective of study
- 1.5Limitation of study
- 1.6Scope of study
- 1.7Significance of study
- 1.8Structure of the research
- 1.9Definition of terms
Chapter TWO
LITERATURE REVIEW
- 2.1Overview of Phytochemicals
- 2.2Importance of Phytochemical Analysis
- 2.3Cleome Viscosa: Botanical Description
- 2.4Phytochemical Composition of Cleome Viscosa
- 2.5Methods for Phytochemical Extraction
- 2.6Analytical Techniques for Phytochemical Analysis
- 2.7Health Benefits of Phytochemicals
- 2.8Phytochemicals in Traditional Medicine
- 2.9Challenges in Phytochemical Analysis
- 2.10Future Trends in Phytochemical Research
Chapter THREE
RESEARCH METHODOLOGY
- 3.1Research Design
- 3.2Sampling Methods
- 3.3Data Collection Procedures
- 3.4Data Analysis Techniques
- 3.5Ethical Considerations
- 3.6Research Validity and Reliability
- 3.7Research Limitations
- 3.8Research Assumptions
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- 4.1Overview of Research Findings
- 4.2Phytochemical Analysis of Cleome Viscosa
- 4.3Comparison with Existing Studies
- 4.4Interpretation of Results
- 4.5Implications of Findings
- 4.6Recommendations for Further Research
- 4.7Practical Applications of Findings
- 4.8Conclusion
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- 5.1Summary of Findings
- 5.2Conclusion
- 5.3Contributions to Knowledge
- 5.4Practical Implications
- 5.5Recommendations
- 5.6Areas for Future Research
- 5.7Reflection on Research Process
- 5.8Conclusion
Thesis Abstract
Abstract
Cleome viscosa, commonly known as tickweed or Asian spider flower, is a plant species with a long history of traditional medicinal use in various parts of the world. The plant is known for its potential pharmacological properties due to its rich phytochemical content. This study aimed to conduct a comprehensive phytochemical analysis of Cleome viscosa to identify and quantify its bioactive compounds. Various extraction techniques, including Soxhlet extraction, maceration, and ultrasound-assisted extraction, were employed to obtain different extracts from the plant material. The phytochemical analysis of these extracts was carried out using standard qualitative and quantitative methods. The presence of various phytochemical classes such as alkaloids, flavonoids, phenolics, terpenoids, and saponins was confirmed through preliminary screening tests. Quantitative analysis revealed the total phenolic and flavonoid contents of the extracts, which were found to be significant, indicating the potential antioxidant properties of Cleome viscosa. High-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses were performed to identify specific bioactive compounds present in the extracts. The HPLC analysis detected the presence of quercetin, rutin, and catechin, known for their antioxidant and anti-inflammatory properties. The GC-MS analysis revealed the presence of various compounds such as linoleic acid, stigmasterol, and phytol, which have been reported to possess diverse pharmacological activities including anti-cancer, anti-inflammatory, and antimicrobial properties. The results of this study provide valuable insights into the phytochemical composition of Cleome viscosa, highlighting its potential therapeutic benefits. Furthermore, the antioxidant activity of the extracts was evaluated using different in vitro assays, including DPPH scavenging activity and total antioxidant capacity. The results indicated significant antioxidant potential, further supporting the traditional medicinal uses of Cleome viscosa. In conclusion, the phytochemical analysis of Cleome viscosa revealed the presence of various bioactive compounds with potential pharmacological activities. The antioxidant properties of the plant extracts suggest their possible application in the development of natural antioxidant and therapeutic agents. Further studies are warranted to explore the specific mechanisms of action and clinical applications of these phytochemicals for potential drug development and health benefits.
Thesis Overview
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</p><div><p><strong>1.0 INTRODUCTION</strong></p><p><strong>Definition</strong></p><p>The <em>Cleome viscosa Linn</em> is commonly known as Asian spider flower or yellow spider flower. It belongs to Capperaceae family<em>.C.viscosa</em> is a weed distributed throughout the tropics of the world and the plains of india. It is known as Asian spider flower in English, Namijin `yaranguwa in Hausa, Hurhur in India, Hurhuria in Bengali, Nayikkadugan in Tamil (Asolkar<em>et, al</em>. 1992).Traditionally, this plant is used in various disorders such as diarrhoea, fever, inflammation, liver diseases, bronchitis, skin diseases, and malarial fever (Henty and Pritchard, 1975). The juice is useful in piles, lumbago and earache. The analgesic, antipyretic and anti-diarrhoeal activities of the extract have been reported by researchers, it was noted that the fresh leaves of <em>C.viscosa</em> are widely used as medicine for Jaundice.</p><p>1</p></div><div><p>Taxonomical profile</p><p>Kingdom: Plantea</p><p>Phylum: Tracheophyta</p><p>Class: Magnoliopsida</p><p>Order: Capparales</p><p>Family: Capparaceae</p><p>Genus: Cleome</p><p>Specie: Viscosa</p><p>(UNEP-WCMC 2011)</p><p><strong>Brief description of the plant</strong></p><p><em>Cleome viscosa </em>belonging to the family Capparaceae (Narayana<em>et, al., </em>2003). It is an annual sticky herb with strong penetrating odor. The plant is clothed with glandular and simple hairs (Rukmini, 1978). It is a widely distributed herbaceous plant with yellow flowers and long slender pods containing seeds, which is similar to that of mustard. <em>C.viscosa</em>, also called “Dog mustard”, is a herb that</p></div><div><p>grow up to 1m height in India (Parimala<em>et, al.,</em> 2004). Cleome is a large genus included in the Capparaceae family, which comprises 427 species occurring in tropical and subtropical regions of the world (Brummit, 1992). The native of the plant is Africa and S. Arabia to Australia.The leaves are diaphoretic, rubefacient and vesicant. They are used as an external application to wounds and ulcers. The juice of the leaves has been used to relieve earache. The seeds are anthelmintic, carminative, rubefacient and vesicant. The seeds of <em>C.viscosa</em> are used to treat fever, diarrhea, and infantile convulsion (Rashmi<em>, et al.,</em> 2012). <em>C.viscosa</em> is a popular remedy for a variety of ailments as documented in Ethno-botanical surveys and traditional systems of medicine, such as Ayurveda, Siddha and Unani (Raghavan, 1993).</p><p><strong>1.1 Importance of phytochemicals</strong></p><p>The defuttered extract were tested for the presence of different secondary plant metabolites. Basic phytochemical screening consist of performing simple chemical test to detect the presence of alkanoids (Herbone, 1973),Tanins (Herbone, 1998) and saponid and cardial glycoside.</p></div><div><p>Observation of antibacterial activity may be due to the presence of potent phytochemical constituents in the extracts. The data obtained in the present work will be useful in the synthesis of new drugs of pharmaceutical importance. Phytochemical analysis or screening can be useful to substantiate and authenticate drugs e.gpharmacognostic</p><p><strong>1.2 Statement of the problem</strong></p><p>The chemical pollution of soil has become a major source of concern and has posed serious health problem within the last few years in many developed and developing nations (Ahmadpour<em>et, al.,</em> 2010). The effect of heavy metal on plant resulted inhibition, structural damage, a decline of physiological and biochemical activities, as well as of the function of the plant (Oancea, 2005)<strong>.</strong> As a weed, <em>C.viscosa</em>is found in abundance within the University premises including students hostels and staff quarters, road-site, University mini market and other areas. The plant is increasingly dominating agricultural lands and hence pose danger to other plant species.</p></div><p><strong>1.3 Justification</strong></p><p>This plant specie is considered as a weed. Phytochemicals are generally used as a essential nutrient responsible for the protection and carrying of many health situation, including fever, diarrhea, infantile convulsion, ulcer and earache e.t.c. Hence it is important to determine the specie chemical compositions present in the leaf, pod and roots.</p><p><strong>1.4 Aim of the study</strong></p><p>The aim of the study is to determine and identify some phytochemicals present in the leaf, root and pods of <em>C. viscosa.</em></p><p><strong>1.5 objectives of the study</strong></p><p>This study have the following objectives;</p><p>1. To determine phytochemicals presence in the root, leaves and pods of <em>C. viscosa.</em></p><p>2. To evaluate the difference concentrations of the secondary metabolites in the root, leaves and pods of <em>C. viscosa</em>.</p>
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