The Purpose of this study was to develop and standardize a Chemistry achievement test by applying Item response theory. Instrumentation research design was used for the study and the area of study was Umuahia education zone in Abia state. The population of the study comprised of all senior secondary two (SS11) students of 2013/ 2014 academic session in this education zone and a sample of 1035 students were drawn through multi – stage sampling procedure from the population. The instrument used for data collection was Chemistry achievement test (CAT), and to ensure the validity of the instrument, the instrument was subjected to face and content validation by three experts from science education department and one senior chemistry teacher. The reliability index of 0.74 was obtained. The data collected with CAT were analyzed using BILOG – MG computer program. The findings of the study showed that forty eight (48) items of the Chemistry achievement test were difficult and fourteen (14) items discriminated moderately. The findings further revealed that the ability estimates of the male students and female students did not function differentially. Based on the findings, recommendations were made which include that the examination bodies and teachers will be encouraged to adopt IRT in developing test items used in measuring students’ ability in Chemistry.
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