The phytochemical constituents of hexanolic extract of ocimum gratissimum (scent leaves) | Blazingprojects Postgraduate Thesis
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The phytochemical constituents of hexanolic extract of ocimum gratissimum (scent leaves)

 

Table Of Contents


  • 1.0    Introduction1.1     Aim of Study1.2     Literature Review1.3     Phytochemicals1.
  • 3.1Alkaloids1.
  • 3.2Flavonoids1.3.
  • 2.1Antioxidant activities1.3.
  • 2.2Antimicrobial activities1.3.
  • 2.3Antibacterial activities1.3.
  • 2.3Antiatherosclerotic effects1.
  • 3.3Saponins1.
  • 3.4TanninsCHAPTER TWO2.0     Materials and Methods2.1     Materials Used2.2     Collection and Identification of samples2.3     Sample preparation2.
  • 3.1Drying of the leaves2.
  • 3.2    Pulverisation of the leaves2.
  • 3.3    Extraction Procedure2.
  • 3.4    Filtration Process2.
  • 3.5    Concentration Process2.4       Phytochemical Analysis2.
  • 4.1  Oualitative Analysis2.4.
  • 1.1Test for Alkaloids2.4.
  • 1.2Test for Phytosterol2.4.
  • 1.3Test for Flavonoids2.4.
  • 1.4Test for Saponin2.4.
  • 1.5Test for Phenol and Tannin2.
  • 4.2    Ouantitative Analysis2.4.
  • 2.1Determination of flavonoid content2.4.
  • 2.2Determination of tannins content2.4.
  • 2.3Determination of Saponins content2.4.
  • 2.4Determination of flavonoid content2.4.
  • 2.5Determination of Phenolic content2.4.
  • 2.6Determination of Reducing PowerCHAPTER THREE3.0     ResultsCHAPTER FOUR4.0     Discussion and Conclusion4.1     Discussion4.2     ConclusionReferencesLIST OF TABLESTable 1 – Showing the qualitative analysis results of phytochemical constituents of Ocimum grattissimum                                                    Table 2 – Showing the quantitative analysis of phytochemical constituents of Ocimum grattissimum Table 3 – Showing the flavonoids determinationTable 4 – Showing the tannin determinationTable 5 – Showing the total phenol determinationTable 6 – Showing the ascorbic acid absorbanceTable 7 – Bioactivity of compounds detected through GC-MS screening of methanolic extract of scent leavesLIST OF FIGURESFigxure 1 – Graph showing the flavonoids determinationFigure 2 – Graph showing the tannin determinationFigure 3 – Graph showing the total Phenol determinationFigure 4 – Graph showing the reducing standard curve.

Thesis Abstract

Abstract
Ocimum gratissimum, commonly known as scent leaves, is a plant with various traditional medicinal uses due to its rich phytochemical composition. In this study, the hexanolic extract of Ocimum gratissimum was analyzed to identify its phytochemical constituents. The extraction process was carried out using hexane as the solvent, followed by evaporation to obtain the concentrated hexanolic extract. The extract was then subjected to qualitative phytochemical screening tests to determine the presence of various classes of phytochemicals such as alkaloids, flavonoids, tannins, saponins, phenols, and terpenoids. The results of the phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, saponins, phenols, and terpenoids in the hexanolic extract of Ocimum gratissimum. Alkaloids are known for their pharmacological properties such as analgesic and anti-inflammatory effects. Flavonoids possess antioxidant and anti-inflammatory activities, while tannins exhibit antimicrobial and antioxidant properties. Saponins have been reported to have anticancer and cholesterol-lowering effects. Phenols are antioxidants that can help in preventing various diseases, and terpenoids have shown antimicrobial and anti-inflammatory activities. The identification of these phytochemical constituents in the hexanolic extract of Ocimum gratissimum highlights the potential medicinal properties of this plant. The presence of alkaloids, flavonoids, tannins, saponins, phenols, and terpenoids suggests that the extract may possess various pharmacological activities such as antioxidant, anti-inflammatory, antimicrobial, and anticancer effects. These bioactive compounds contribute to the therapeutic properties of Ocimum gratissimum and support its traditional uses in herbal medicine. Further studies are recommended to isolate and characterize the individual phytochemical compounds present in the hexanolic extract of Ocimum gratissimum. Understanding the specific compounds responsible for the observed pharmacological activities will provide valuable insights for the development of new therapeutic agents from this plant. Additionally, bioassays should be conducted to evaluate the biological activities of the extract and its isolated compounds in order to validate their potential medicinal applications.

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