The oxidative stress status of rats fed on oil bean seed

Thesis Abstract

Abstract
Oil bean seeds are commonly used in animal feed due to their high protein and energy content. However, concerns have been raised regarding the potential oxidative stress that may arise from the consumption of oil bean seeds due to their high levels of anti-nutritional factors. This study aimed to investigate the oxidative stress status of rats fed on oil bean seeds. Thirty male Wistar rats were divided into three groups a control group fed a standard diet, a low-dose group fed a diet containing 10% oil bean seeds, and a high-dose group fed a diet containing 20% oil bean seeds. After six weeks, blood and tissue samples were collected for analysis. Results showed that rats in the high-dose group had significantly higher levels of oxidative stress markers such as malondialdehyde (MDA) and decreased levels of antioxidant enzymes including superoxide dismutase (SOD) and glutathione peroxidase (GPx) compared to the control group. Histopathological examination of liver and kidney tissues revealed signs of oxidative damage in the high-dose group, including lipid peroxidation and cellular degeneration. Furthermore, the high-dose group exhibited a significant increase in pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-?) and interleukin-6 (IL-6) compared to the control group, indicating an inflammatory response associated with oxidative stress. Interestingly, the low-dose group did not show significant differences in oxidative stress markers compared to the control group, suggesting a dose-dependent effect of oil bean seed consumption on oxidative stress status in rats. In conclusion, the results of this study demonstrate that high dietary intake of oil bean seeds can lead to oxidative stress and inflammation in rats, potentially due to the presence of anti-nutritional factors in the seeds. Further research is needed to elucidate the mechanisms underlying the oxidative stress induced by oil bean seeds and to explore potential strategies to mitigate these effects in animal feed formulations.