Phytochemical analysis of cleome viscosa. | Blazingprojects Postgraduate Thesis
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Phytochemical analysis of cleome viscosa.

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Phytochemicals
  • 2.2Importance of Phytochemical Analysis
  • 2.3Methods for Phytochemical Analysis
  • 2.4Phytochemicals in Cleome Viscosa
  • 2.5Health Benefits of Phytochemicals
  • 2.6Role of Phytochemicals in Plants
  • 2.7Factors Affecting Phytochemical Composition
  • 2.8Phytochemical Extraction Techniques
  • 2.9Analysis of Phytochemical Compounds
  • 2.10Recent Advances in Phytochemical Research

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Design
  • 3.2Selection of Research Methods
  • 3.3Data Collection Procedures
  • 3.4Sampling Techniques
  • 3.5Data Analysis Methods
  • 3.6Research Ethics
  • 3.7Research Validity and Reliability
  • 3.8Limitations of the Research Methodology

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Overview of Research Findings
  • 4.2Phytochemical Analysis Results
  • 4.3Comparison with Existing Studies
  • 4.4Interpretation of Findings
  • 4.5Implications of Findings
  • 4.6Recommendations for Future Research
  • 4.7Practical Applications of Findings
  • 4.8Areas for Further Exploration

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Findings
  • 5.2Conclusion
  • 5.3Contributions to the Field
  • 5.4Recommendations for Practice
  • 5.5Suggestions for Future Research

Thesis Abstract

Abstract
Cleome viscosa, commonly known as Asian spider flower or tickweed, is a plant with significant medicinal properties. In this study, phytochemical analysis of Cleome viscosa was conducted to identify and quantify the bioactive compounds present in the plant. Various extraction techniques including maceration, Soxhlet extraction, and ultrasound-assisted extraction were employed to extract the phytochemicals from the plant material. The phytochemical analysis revealed the presence of a variety of bioactive compounds such as alkaloids, flavonoids, phenols, tannins, saponins, and terpenoids in Cleome viscosa. Quantitative analysis using spectrophotometric methods indicated the levels of these compounds in different parts of the plant. The highest concentrations of alkaloids and flavonoids were found in the leaves, while the stems showed higher levels of phenols and tannins. Saponins were predominantly present in the roots, and terpenoids were distributed throughout the plant. Further characterization of the bioactive compounds was performed using chromatographic techniques such as HPLC and GC-MS. The results indicated the presence of specific compounds such as quercetin, rutin, catechin, and ?-sitosterol in Cleome viscosa extracts. These compounds have been reported to exhibit various pharmacological activities including antioxidant, anti-inflammatory, antimicrobial, and anticancer properties. The antioxidant activity of Cleome viscosa extracts was evaluated using DPPH and ABTS radical scavenging assays. The results demonstrated significant antioxidant potential, attributed to the presence of flavonoids and phenolic compounds in the plant extracts. The antimicrobial activity of the extracts was assessed against a panel of pathogenic microorganisms, showing moderate to potent inhibitory effects. Overall, the phytochemical analysis of Cleome viscosa revealed its rich chemical composition and diverse bioactive properties. The findings of this study provide valuable insights into the potential medicinal applications of Cleome viscosa as a natural source of bioactive compounds with therapeutic benefits. Further research is warranted to explore the specific mechanisms of action and potential pharmaceutical applications of the identified compounds for the development of novel drugs and functional foods.

Thesis Overview

<p> </p><div><p><strong>1.0 INTRODUCTION</strong></p><p><strong>Definition</strong></p><p>The <em>Cleome viscosa Linn</em>&nbsp;is commonly known as Asian spider flower or yellow spider flower. It belongs to Capperaceae family<em>.C.viscosa</em>&nbsp;is a weed distributed throughout the tropics of the world and the plains of india. It is known as Asian spider flower in English, Namijin `yaranguwa in Hausa, Hurhur in India, Hurhuria in Bengali, Nayikkadugan in Tamil (Asolkar<em>et, al</em>. 1992).Traditionally, this plant is used in various disorders such as diarrhoea, fever, inflammation, liver diseases, bronchitis, skin diseases, and malarial fever (Henty and Pritchard, 1975). The juice is useful in piles, lumbago and earache. The analgesic, antipyretic and anti-diarrhoeal activities of the extract have been reported by researchers, it was noted that the fresh leaves of <em>C.viscosa</em>&nbsp;are widely used as medicine for Jaundice.</p><p>1</p></div><div><p>Taxonomical profile</p><p>Kingdom: Plantea</p><p>Phylum: &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Tracheophyta</p><p>Class: &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Magnoliopsida</p><p>Order: &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Capparales</p><p>Family: &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Capparaceae</p><p>Genus: &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Cleome</p><p>Specie: &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Viscosa</p><p>(UNEP-WCMC 2011)</p><p><strong>Brief description of the plant</strong></p><p><em>Cleome viscosa </em>belonging to the family Capparaceae (Narayana<em>et, al., </em>2003). It is an annual sticky herb with strong penetrating odor. The plant is clothed with glandular and simple hairs (Rukmini, 1978). It is a widely distributed herbaceous plant with yellow flowers and long slender pods containing seeds, which is similar to that of mustard. <em>C.viscosa</em>, also called “Dog mustard”, is a herb that</p></div><div><p>grow up to 1m height in India (Parimala<em>et, al.,</em>&nbsp;2004). Cleome is a large genus included in the Capparaceae family, which comprises 427 species occurring in tropical and subtropical regions of the world (Brummit, 1992). The native of the plant is Africa and S. Arabia to Australia.The leaves are diaphoretic, rubefacient and vesicant. They are used as an external application to wounds and ulcers. The juice of the leaves has been used to relieve earache. The seeds are anthelmintic, carminative, rubefacient and vesicant. The seeds of <em>C.viscosa</em>&nbsp;are used to treat fever, diarrhea, and infantile convulsion (Rashmi<em>, et al.,</em>&nbsp;2012). <em>C.viscosa</em>&nbsp;is a popular remedy for a variety of ailments as documented in Ethno-botanical surveys and traditional systems of medicine, such as Ayurveda, Siddha and Unani (Raghavan, 1993).</p><p><strong>1.1 Importance of phytochemicals</strong></p><p>The defuttered extract were tested for the presence of different secondary plant metabolites. Basic phytochemical screening consist of performing simple chemical test to detect the presence of alkanoids (Herbone, 1973),Tanins (Herbone, 1998) and saponid and cardial glycoside.</p></div><div><p>Observation of antibacterial activity may be due to the presence of potent phytochemical constituents in the extracts. The data obtained in the present work will be useful in the synthesis of new drugs of pharmaceutical importance. Phytochemical analysis or screening can be useful to substantiate and authenticate drugs e.gpharmacognostic</p><p><strong>1.2 Statement of the problem</strong></p><p>The chemical pollution of soil has become a major source of concern and has posed serious health problem within the last few years in many developed and developing nations (Ahmadpour<em>et, al.,</em>&nbsp;2010). The effect of heavy metal on plant resulted inhibition, structural damage, a decline of physiological and biochemical activities, as well as of the function of the plant (Oancea, 2005)<strong>.</strong>&nbsp;As a weed, <em>C.viscosa</em>is found in abundance within the University premises including students hostels and staff quarters, road-site, University mini market and other areas. The plant is increasingly dominating agricultural lands and hence pose danger to other plant species.</p></div><p><strong>1.3 Justification</strong></p><p>This plant specie is considered as a weed. Phytochemicals are generally used as a essential nutrient responsible for the protection and carrying of many health situation, including fever, diarrhea, infantile convulsion, ulcer and earache e.t.c. Hence it is important to determine the specie chemical compositions present in the leaf, pod and roots.</p><p><strong>1.4 Aim of the study</strong></p><p>The aim of the study is to determine and identify some phytochemicals present in the leaf, root and pods of <em>C. viscosa.</em></p><p><strong>1.5 objectives of the study</strong></p><p>This study have the following objectives;</p><p>1. &nbsp; To determine phytochemicals presence in the root, leaves and pods of <em>C. viscosa.</em></p><p>2. &nbsp; &nbsp; To evaluate the difference concentrations of the secondary metabolites in the root, leaves and pods of <em>C. viscosa</em>.</p> <br><p></p>

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