Isolation, identification and antibiogram of staphylococcus aureus isolated from cow meat | Blazingprojects Postgraduate Thesis
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Isolation, identification and antibiogram of staphylococcus aureus isolated from cow meat

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of study
  • 1.3Problem Statement
  • 1.4Objective of study
  • 1.5Limitation of study
  • 1.6Scope of study
  • 1.7Significance of study
  • 1.8Structure of the research
  • 1.9Definition of terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Staphylococcus aureus
  • 2.2History of Staphylococcus aureus
  • 2.3Morphology and characteristics
  • 2.4Pathogenesis and diseases caused
  • 2.5Transmission and control measures
  • 2.6Antibiotic resistance mechanisms
  • 2.7Detection and identification methods
  • 2.8Epidemiology of Staphylococcus aureus
  • 2.9Foodborne transmission of Staphylococcus aureus
  • 2.10Impact on public health

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research design
  • 3.2Sampling techniques
  • 3.3Data collection methods
  • 3.4Laboratory procedures
  • 3.5Data analysis techniques
  • 3.6Ethical considerations
  • 3.7Research limitations
  • 3.8Research validity and reliability

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Descriptive analysis of Staphylococcus aureus isolates
  • 4.2Antibiotic susceptibility testing results
  • 4.3Comparison with existing literature
  • 4.4Factors influencing antibiotic resistance
  • 4.5Discussion on implications for public health
  • 4.6Recommendations for future research
  • 4.7Limitations of the study
  • 4.8Conclusion of the findings

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of findings
  • 5.2Conclusion
  • 5.3Practical implications
  • 5.4Contribution to knowledge
  • 5.5Recommendations for practice
  • 5.6Recommendations for policy
  • 5.7Suggestions for further research

Thesis Abstract

The study was carried out with aim to isolate Staphylococcus aureus from cow meat and determine the antibiogram pattern of S. aureus. Three samples of cow meat from three different locations (Gwagwalada market, Abattoir market and Kasuan Dare) in Gwagwalada were collected to isolate S. aureus from the samples. The organisms isolated were Salmonella spp, Escherichia coli, Staphylococcus aureus and Listeria monocytogenes. Several biochemical tests were carried out to identify, the S. aureus isolates like Catalse test (positive), Manitol test (positive), Coagulase test (positive) and Oxidase test (negative). The antibiogram pattern of S. aureus was evaluated using commercially prepared disk. The antibiotics tested were Pefloxacin (Pef), Streptomycin (S), Ampiclox (Apx), Amoxacilin (Am) and Ciprofloxacin. The pattern indicated that the overall S. aureus were resistant to Pef, S, Cpx and less resistant to Am and Apx. Results clearly suggested a possibility of potential public health threat of S. aureus resulting from contamination of cow meat with pathogenic bacteria which is mainly due to unhygienic processing, handling, and unhygienic environment.

Thesis Overview

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