Isolation, characterisation and antimicrobial activity of bioactive constituents from the leaf extract of adenodolichos paniculatus (hua) hutch. & dalz (fabaceae)

Thesis Abstract

Abstract
Adenodolichos paniculatus (Hua) Hutch. & Dalz (Fabaceae) is a plant with traditional medicinal uses in various regions. This study focused on the isolation, characterization, and antimicrobial activity evaluation of bioactive constituents from the leaf extract of A. paniculatus. The extraction process involved the use of appropriate solvents to obtain the crude extract. Various chromatographic techniques such as column chromatography, thin-layer chromatography, and high-performance liquid chromatography were employed for the isolation and purification of the bioactive compounds. Characterization of the isolated compounds was carried out using spectroscopic methods including UV-Vis, FTIR, NMR, and MS. These techniques provided valuable information about the chemical structures of the bioactive constituents present in the leaf extract. The antimicrobial activity of the isolated compounds was evaluated against a panel of pathogenic microorganisms including bacteria and fungi. The agar well diffusion method and microdilution technique were used to determine the minimum inhibitory concentration (MIC) and zone of inhibition of the compounds. The results of the study revealed the presence of bioactive compounds in the leaf extract of A. paniculatus with significant antimicrobial properties. The isolated compounds exhibited varying degrees of antimicrobial activity against the tested microorganisms. The MIC values indicated the concentration at which the compounds effectively inhibited the growth of the microorganisms. Furthermore, the zone of inhibition results demonstrated the ability of the compounds to prevent the growth of the pathogens in the agar medium. Overall, this study provides valuable insights into the bioactive constituents present in the leaf extract of A. paniculatus and their antimicrobial potential. The isolation and characterization of these compounds contribute to the understanding of the plant's therapeutic properties and may have implications for the development of novel antimicrobial agents. Further research is warranted to explore the mechanisms of action of the bioactive compounds and their potential applications in the pharmaceutical industry.