Effect of different carbon sources on the growth of antimicrobial producing bacillus species in citrillus vugaris. | Blazingprojects Postgraduate Thesis
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Effect of different carbon sources on the growth of antimicrobial producing bacillus species in citrillus vugaris.

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Carbon Sources
  • 2.2Growth Factors in Bacillus Species
  • 2.3Antimicrobial Production in Bacillus
  • 2.4Citrullus Vulgaris as a Host Plant
  • 2.5Effect of Carbon Sources on Microbial Growth
  • 2.6Influence of Carbon Sources on Antimicrobial Production
  • 2.7Interactions between Bacillus Species and Citrullus Vulgaris
  • 2.8Importance of Studying Carbon Sources in Microbial Growth
  • 2.9Comparative Analysis of Different Carbon Sources
  • 2.10Research Gaps in Carbon Source Studies

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Design
  • 3.2Sampling Methods
  • 3.3Data Collection Techniques
  • 3.4Experimental Setup
  • 3.5Statistical Analysis Methods
  • 3.6Variables and Controls
  • 3.7Ethical Considerations
  • 3.8Data Interpretation Techniques

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Analysis of Growth Patterns
  • 4.2Antimicrobial Production Levels
  • 4.3Impact of Carbon Sources on Bacillus Growth
  • 4.4Relationship Between Carbon Sources and Antimicrobial Activity
  • 4.5Comparison of Growth in Different Carbon Sources
  • 4.6Influence of Citrullus Vulgaris on Bacillus Species
  • 4.7Interpretation of Experimental Results
  • 4.8Discussion on the Findings

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Research Findings
  • 5.2Conclusion
  • 5.3Recommendations for Future Studies
  • 5.4Practical Implications
  • 5.5Contribution to the Field
  • 5.6Limitations of the Study
  • 5.7Final Thoughts
  • 5.8References

Thesis Abstract

Effect of different carbon sources on the growth of antimicrobial producing Bacillus species in ogiri-egusi sold in Ogbeta, Orie, Artisian, Kenyatta, Enugu State were evaluated using standard microbiological and analytical methods. 12 bacteria were isolated and identified such as Bacillus subtilis,(33.33), Bacillus pumulis (41.66), Bacillus licheniformis (25) were identified based on their colony biochemical characteristics.The isolates of the highest occurrences is bacillus pumilus. Agar well diffusion method was used to determine the antimicrobial activity against two pathogenic organisms, Escherichia coli, Staphylococcus aureus. Bacillus subtilis and Bacillus pumulis, gave a zone of inhibition Escherichia coli 0.6mm and 0.8mm after 24 hours of incubation also carbon sources of 5% and 10% sucrose, Glucose and soluble starch were used to check their different carbon sources on the isolates using the spectrophotometer.5% had the highest optimum growth of 1.400 for glucose 1.545 for sucrose,2.376 for soluble starch which can be added in maximum quantity to a medium to produce antibiotics.

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