COMBINED EFFECT OF ETHANOL EXTRACT OF MUCUNA URENS (HORSE-EYE BEANS SEED) AND ETHANOL EXTRACT OF ZINGIBER OFFICINALE (GINGER RHIZOME) ON SPERM MOTILITY IN MALE WISTAR ALBINO RATS

Thesis Overview

<p> </p><p><strong>1.1 &nbsp; &nbsp; &nbsp; Background of the Study</strong></p><p>The understanding of male reproductive function and the importance of male factor in infertility has advanced significantly in the recent time. The reproductive function of male is divided into three major subdivisions: spermatogenesis, performance of the male sexual act and regulation of male reproductive functions by the various hormones. Association with these function are the effects of male sex hormones on the accessory sexual organs, cellular metabolism, growth and other body functions (Guyton and Hall, 2011)<em>. </em>Infertility is a disease of reproductive system defined by failure to achieve the clinical pregnancy after 12 months or more of regular unprotected sexual intercourse (Zegers-Hochschild <em>et al.,</em>&nbsp;2009). Infertility is one of the major health challenges in life approximately 30% of infertility is due to male factor <em>(</em>Isidori<em>&nbsp;et al., 2006).</em></p><p>Factors affecting male infertility are classified into pre-testicular, testicular and post-testicular <em>(</em>Balen,2008<em>).</em>&nbsp;This may cause the testicles producing a decreased number of sperm or disturbance along the path preventing cells from maturing into sperm production or reaching the woman fallopian tube where fertilization occur. Germ cells are gradually transformed into spermatozoa. This occurs in the seminferous tubules of the testes. It is divided into three stages <em>(</em>Cuninghan and Kelvin, 2007<em>)</em>. Proliferative phase: Spermatogonia-Spermatocytes, Meiotic phase: spermatocytes – spermatids, Differentiation phase: (Spermiogenesis) spermatids-spermatozoa <em>(</em>Hess, 1999<em>).</em>Several conditions interfere with spermatogenesis and reduce sperm quality and production,</p><p>Semen is an organic fluid that may contains spermatozoa. It is secreted by the gonads (sexual glands). Seminal fluid contains spermatozoa, proteolytic enzymes and fructose which promote the survival of spermatatozoa and provide a medium through which they move or swim <em>(</em>Ali<em>&nbsp;et al., 2015)</em>. Male infertility can be assess through semen analysis and reproductive hormonal profile <em>(</em>Jungwirth<em>, et al</em>., 2012<em>)</em>. Male infertility is commonly due to deficiencies in the semen and semen quality <em>(</em>Cooper<em>&nbsp;et al.,</em>2010<em>).</em></p><p>Males with sperm parameters below the WHO normal values are considered to have male factor infertility. Most significant of these are low sperm concentration (oligospermia), poor sperm motility (asthenospermia), and abnormal sperm morphology (tetratospermia). Factors less associated with infertility include semen volume and other serminal markers of epididymal, prostatic and serminal vesicle function <em>(</em>Harris<em>&nbsp;et al.,</em>2011<em>).</em>&nbsp;Positive association exit between abnormal semen parameters and sperm count <em>(</em>Sebra<em>&nbsp;et al., 2014)</em>.</p><p>Abnormality in sperm count, motility and morphology arises from disarray in control mechanism, including pre-testicular, testicular and post testicular factors <em>(</em>Wamoto<em>&nbsp;et al.,</em>2007). Semen analysis remains the single most useful and fundamental investigation to detect men with a genuine problem of male infertility (Butt<em>&nbsp;et al.,</em>2013). It is a simple test that assesses the formation and maturity of sperm as well as how the sperm interacts in the serminal fluid. It also provides insight not only on sperm production (count), but the sperm quality (motility and morphology) as well <em>(</em>Fisch,2008<em>)</em>. As many as 2% men exhibits suboptimal sperm parameters, which may be one or a combination of low sperm concentration, poor sperm motility, or abnormal morphology (Kumar<em>&nbsp;et al.,</em>2015<em>)</em>.</p><p><em>Mucuna urens</em>&nbsp;also known as Ox-eye beans is a common soup thickener consumed mostly in South Eastern states in Nigeria, it is a plant belonging to the family fabaceae, commonly found in home and gardens in the south eastern part of Nigeria, West Africa, where the Efiks, Ibibio and Igbos uses the seed as a major soup condiment for thickening (Achivewhu, 1984). It is incorporated into the normal feed for farm animals in the North due to its protein content <em>(</em>Umoren<em>&nbsp;et al.,</em>&nbsp;2007). Mucuna urens is called “Ibaba” by the Efik/Ibibios and “Ukpor” by the Igbos and usually sold in the local markets during the harvest season ie January <em>(</em>Eilitta <em>et al.,</em>&nbsp;2003). It may be found throughout the year. Mucuna urens requires support for growth hence cultivated near trees. This enables production of many seeds per plant <em>(</em>Sridhar<em>et al.,</em>2007). Other names for Mucuna urensinclude Horse eye bean, Ox-eye bean and devil bean. Mucuna urens had been found to contain some endogenous toxic factors. High concentration of tannis, phytic acid, cynogenic glucoside, oxalate and gossypol has been reportedin Mucuna urens (Laurena<em>&nbsp;et al., 1994).</em></p><p>Toxic compounds including L-DOPA (3,4-dihydroxy-L-phenylalanine), nicotine, physostigmine and serotonine are found in Mucuna urens, which also act as anti-nutritive value of Mucuna urens (Umoren<em>&nbsp;et al.,</em>2007)<em>. Mucuna urens</em>&nbsp;seed extract causes sperm degeneration in testicular tubules,collapse of villi in the prostate gland and seminal vesicles of male guinea-pigs <em>(</em>Udoh and Ekpeyong, 2001).</p><p>Ginger rhizome (<em>Zingiber officinale Roscoe</em>; Family: <em>Zingibercease)</em>&nbsp;is used worldwide as a spice. It has both anti-oxidative and androgenic activities in animal models (Sekiwa <em>et al.,</em>2000, Kantch Oung<em>&nbsp;et al., 2002).</em>&nbsp;The local names of ginger includes; ‘Afa-ije’ in Yoruba and ‘Jinja’ in Igbo/Efik/Ibibios. Ginger rhizome contains active ingredients such as Zingerone, Gingerdiol, Zingibren,Gingerol and shogaols which have antioxidants activity (Zulican<em>&nbsp;et al.,</em>2002). Ginger is a great source of manganese, essential to man’s sex drive and sperm count by helping the body produce testosterone. Ginger oil has protective effect on DNA damage induced by H2O2 and might be used as antioxidant (Grzarana<em>&nbsp;et al., </em>2005). Antioxidant protects DNA and other important molecules from oxidation and damage which can improve sperm quality and consequently increase fertility rate in men <em>(</em>Rajeer<em>&nbsp;et al., </em>2006).</p><p>Ginger is a native of South Eastern Asia but over the centuries has been spread to various part of the world including Africa. In Nigeria, the highest producer of ginger is Kaduna state, others includes Gombe, Bauchi, Benue, Nassarawa among others. Ginger is available in various forms; Fresh ginger rhizome, powder ginger and dry ginger rhizome. A mature ginger rhizome is fibrous and has a striated texture. The outer skin of the rhizome is brownish in colour while the inner flesh depending on the variety may be red, yellow or white. For effective cultivation of ginger the following conditions are required; mulched fertile soil, loam is the most preferred soil type, ridges and a minimum annual rainfall of about 1500mm. An average temperature of about 300C, viable ginger rhizomes with buds and good drainage prevents water logging/flooding.</p> <br><p></p>