The effect of sodium citrate and reduced glutathione (gsh) on arginase from human fibriod tissue
Table Of Contents
- Abstract CHAPTER ONE1.0 Introduction and Literature Review1.1 Fibroid1.
- 1.1 Location and classification of fibroid1.
- 1.2 Pathogenesis and Pathophysiology1.1.
- 2.1 Pathogenesis1.1.
- 2.2 Pathophysiology1.
- 1.3 Signs, Symptoms and Causes of fibroid1.2 Arginase1.
- 2.1 Role of arginase in human body1.
- 2.2 Aginase activities in Benign tumour1.
- 2.3 Arginase II expression in benign and malignant prostate tissue1.3 Glutathione (GSH)1.
- 3.1 Role of glutathione as an antioxidant1.
- 3.2 Glutathione response to exercise1.4 Sodium citrate1.5 Aims and objectivesCHAPTER TWO2.0 Materials and Methods2.1 Materials2.
- 1.1 Apparatus2.2 Methods2.
- 2.1 Preparation of buffers and reagents2.3 Biochemical test2.
- 3.1 Arginase assay2.
- 3.2 Protein standard curve2.
- 3.3 Effect of Glutathione (GSH)CHAPTER THREE3.0 ResultsCHAPTER FOUR4.0 Discussion, Conclusion and Recommendation4.1 Discussion4.2 Conclusion4.3 RecommendationsReferencesLIST OF FIGURESFigure 1 – Uterine FibroidFigure 2 – Hydrolysis of L-arginineFigure 3 – Structure of arginase and the active siteFigure 4 – Showing the effect of sodium citrate on arginase from human fibroid tissueFigure 5 – Showing the effect of Glutathione (GSH) on arginase from human fibroid tissue.
Thesis Abstract
The research is aimed at investigating the effect of sodium citrate and reduced glutathione (GSH) on arginase from human fibriod tissue.
The research shows that arginase enzyme which was partially purified was found to have a specific interaction with its substrate.
The study shows that varying the substrate concentration at constant volume of Sodium citrate and Reduced glutathione (GSH) resulted into an Un-competitive inhibition and Competitive inhibition of enzymes respectively.
The results obtained from this study shows that the inhibition nature of arginase by different inhibitors of enzymes gives a promising approach to abnormal arginase activities in cell proliferation of uterine muscle which leads to fibroid.
Thesis Overview
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