Life cycle assessment of the environmental impact of biosurfactant production from oil waste by a diculture of azotobacter vinelandii and pseudomonas sp.
Table Of Contents
Chapter ONE
INTRODUCTION
- 1.1Introduction
- 1.2Background of Study
- 1.3Problem Statement
- 1.4Objective of Study
- 1.5Limitation of Study
- 1.6Scope of Study
- 1.7Significance of Study
- 1.8Structure of the Research
- 1.9Definition of Terms
Chapter TWO
LITERATURE REVIEW
- 2.1Overview of Biosurfactants
- 2.2Types of Biosurfactants
- 2.3Environmental Impact of Biosurfactant Production
- 2.4Oil Waste as a Raw Material for Biosurfactant Production
- 2.5Azotobacter Vinelandii and Pseudomonas sp. as Biosurfactant Producers
- 2.6Production Processes of Biosurfactants
- 2.7Applications of Biosurfactants
- 2.8Market Trends of Biosurfactants
- 2.9Regulation and Sustainability of Biosurfactant Production
- 2.10Future Prospects in Biosurfactant Research
Chapter THREE
RESEARCH METHODOLOGY
- 3.1Research Methodology Overview
- 3.2Selection of Azotobacter Vinelandii and Pseudomonas sp. as Cultures
- 3.3Collection and Preparation of Oil Waste
- 3.4Biosurfactant Production Process
- 3.5Experimental Design and Parameters
- 3.6Data Collection and Analysis Methods
- 3.7Quality Control Measures
- 3.8Ethical Considerations in Research
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- 4.1Analysis of Biosurfactant Production Efficiency
- 4.2Comparison of Biosurfactant Yields
- 4.3Characterization of Biosurfactants Produced
- 4.4Environmental Impact Assessment
- 4.5Cost Analysis of Biosurfactant Production
- 4.6Market Potential and Economic Feasibility
- 4.7Discussion on Regulatory Compliance
- 4.8Implications and Recommendations for Future Research
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- 5.1Summary of Findings
- 5.2Conclusion
- 5.3Contributions to Knowledge
- 5.4Implications for Industry and Environment
- 5.5Recommendations for Further Research
Thesis Abstract
During the bioprocess of the biosurfactant production (by the consortium), the volume of CO2 evolved was 28.23 ± 5.08 cm3, which is equivalent to 0.056 ± 0.01g CO2 per 100 ml of broth. In terms of gCO2/1000Kg biosurfactant, this gave a value of 4545 ± 817.93g CO2 in this bioprocess. Following from above, the life cycle impact assessment (LCIA) of biosurfactant production by this consortium, based on global warming potential (GWP) was 0.046 tonnes/1000Kg biosurfactant. Other impact values calculated for acidification and eutrophication potentials were 0.008 tonnes / 1000 Kg and 0.0014 tonnes/1000Kg of biosurfactant. These values were considered insufficient in terms of environmental pollution when compared with the regular methods of surfactants production. In this work also, the consortium of Pseudomonas sp/Azotobacter vinelandii produced 1.22 ± 0.04 mg biosurfactant per 100ml of cell – free broth. However, the individual organisms (Pseudomonas sp. and Azotobacter vinelandii) produced 1.03 ± 0.02 and 0.08 ± 0.001 mg of biosurfactants per 100 ml cell – free broth respectively. The microbial growth kinetics during the production of the biosurfactant by the consortium gave a specific maximum growth (µmax) of 1.306 ± 0.201 hr-1), a saturation constant (Ks) of 0.017 ± 0.007 mg/l, an Inhibition constant (Ki) of 121.83 ± 21.18 mg/land a Death constant (Kd) of 0.017 ± 0.006 mg/l. These values when compared with those of individual organisms shows that using a consortium for the bioprocess is more sustainable.
Thesis Overview
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