Isolation, characterisation and antimicrobial activity of bioactive constituents from the leaf extract of adenodolichos paniculatus (hua) hutch. & dalz (fabaceae)
Table Of Contents
Chapter ONE
INTRODUCTION
- 1.1Introduction
- 1.2Background of Study
- 1.3Problem Statement
- 1.4Objective of Study
- 1.5Limitation of Study
- 1.6Scope of Study
- 1.7Significance of Study
- 1.8Structure of the Research
- 1.9Definition of Terms
Chapter TWO
LITERATURE REVIEW
- 2.1Overview of Bioactive Constituents
- 2.2Previous Studies on Adenodolichos Paniculatus
- 2.3Extraction Methods for Bioactive Compounds
- 2.4Characterization Techniques for Bioactive Compounds
- 2.5Antimicrobial Activity of Bioactive Constituents
- 2.6Role of Bioactive Compounds in Medicinal Plants
- 2.7Biological Importance of Antimicrobial Properties
- 2.8Mechanisms of Action of Bioactive Constituents
- 2.9Synergistic Effects of Bioactive Compounds
- 2.10Future Trends in Bioactive Compound Research
Chapter THREE
RESEARCH METHODOLOGY
- 3.1Research Design
- 3.2Sampling Methods
- 3.3Data Collection Procedures
- 3.4Data Analysis Techniques
- 3.5Ethical Considerations
- 3.6Research Instrumentation
- 3.7Statistical Analysis Approaches
- 3.8Validity and Reliability Measures
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- 4.1Overview of Research Findings
- 4.2Bioactive Constituents Identified from Adenodolichos Paniculatus
- 4.3Antimicrobial Activity Results
- 4.4Comparison with Previous Studies
- 4.5Interpretation of Results
- 4.6Discussion on Mechanisms of Action
- 4.7Implications of Findings
- 4.8Recommendations for Further Research
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- 5.1Summary of Findings
- 5.2Conclusions Drawn from the Study
- 5.3Contributions to Existing Literature
- 5.4Practical Applications of Research
- 5.5Limitations of the Study
- 5.6Suggestions for Future Research
- 5.7Conclusion and Final Remarks
Thesis Abstract
The plant Adenodolichos paniculatus which is used in ethno – medicine for the treatment of blennorrhoea, diarrhea, dysentery, small pox, heart burn and wound dressing was investigated for its chemical constituents and antimicrobial activity. The leaves of this plant were subjected to microwave assisted extraction to obtain n – hexane, chloroform, ethyl acetate and methanol extracts. The extracts were then subjected to preliminary phytochemical screening which revealed the presence of alkaloids, anthraquinones, flavonoids, reducing sugars, saponins, tannins and steroids/terpenes. Purification through various column chromatography, the ethyl acetate extract led to the isolation of compounds X1 (mixture of stigmasterol and β – sitosterol) and X2 (nonanoic acid) after spectral analysis IR, 1H NMR and 13C NMR. Determination of inhibitory activity (sensitivity test) using agar well diffusion method showed activity i.e. minimum inhibitory concentration (MIC) against Staphylococcus aureus, Salmonella typhi, and Candida albicans ranging from 12 – 50 mg/ml, Minimum Bactericidal Concentration/ Minimum Fungicidal Concentration (MBC/MFC) ranging from 25 – 50 mg/ml, which is found to be bacteriostatic against Salmonella typhi. Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Bacillus subtili showed no MBC (Bacteriostatic) for the methanol, ethyl acetate, chloroform and n –hexane extract.
Thesis Overview
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