Isolation and purification of 3-mercaptopyruvate sulfurtransferase from the gut of rhinoceros larva (oryctes rhinoceros) biochemistry project topics
Table Of Contents
Chapter ONE
INTRODUCTION
- 1.1Introduction
- 1.2Background of study
- 1.3Problem Statement
- 1.4Objective of study
- 1.5Limitation of study
- 1.6Scope of study
- 1.7Significance of study
- 1.8Structure of the research
- 1.9Definition of terms
Chapter TWO
LITERATURE REVIEW
- 2.1Overview of 3-mercaptopyruvate sulfurtransferase
- 2.2Biological functions of 3-mercaptopyruvate sulfurtransferase
- 2.3Sources of 3-mercaptopyruvate sulfurtransferase
- 2.4Isolation techniques of 3-mercaptopyruvate sulfurtransferase
- 2.5Purification methods of 3-mercaptopyruvate sulfurtransferase
- 2.6Structural characteristics of 3-mercaptopyruvate sulfurtransferase
- 2.7Enzymatic properties of 3-mercaptopyruvate sulfurtransferase
- 2.8Clinical significance of 3-mercaptopyruvate sulfurtransferase
- 2.9Research gaps in the study of 3-mercaptopyruvate sulfurtransferase
- 2.10Summary of literature review
Chapter THREE
RESEARCH METHODOLOGY
- 3.1Research design
- 3.2Sampling techniques
- 3.3Data collection methods
- 3.4Data analysis procedures
- 3.5Ethical considerations
- 3.6Research limitations and challenges
- 3.7Instrumentation used in the study
- 3.8Statistical tools employed in data analysis
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- 4.1Presentation of research findings
- 4.2Analysis of data
- 4.3Comparison of results with existing literature
- 4.4Discussion on experimental outcomes
- 4.5Interpretation of results
- 4.6Implications of findings
- 4.7Recommendations for future research
- 4.8Limitations of the study
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- 5.1Summary of research
- 5.2Conclusions drawn from the study
- 5.3Contributions to the field of biochemistry
- 5.4Practical applications of research findings
- 5.5Recommendations for further studies
Thesis Abstract
Cyanide is known to be one of the most toxic substances present in a wide variety of food materials that are consumed by animals.One of the cyanide detoxifying enzymes is 3-mercaptopyruvate sulfurtransferase (3-MST). Indeed, recent studies have clearly shown that 3-MST is involved in the detoxification of cyanide.Rhinoceros (Oryctes rhinoceros) larva feeds on dead, decayed and living plants, wood and palm. Plants are known to contain cyanide as a defence mechanism for intruding/pesting organisms. Thus, for rhinoceros larva to be able to live on plants, it must have possessed a cyanide-detoxifying enzyme.3-MST, a cyanide-detoxifying enzyme was purified from Rhinoceros (Oryctes rhinoceros) larva in this work.The 3-MST enzyme was isolated from the gut of Oryctes rhinoceros larvae and purified using Ammonium Sulphate Precipitation, Bio-Gel-P-100 Gel Filtration Chromatography and Reactive Blue-2-Agarose Affinity chromatography.The specific activity of the enzyme was 0.22U/mg.The presence of this enzyme could be exploited by including it in the diet of animals which would serve as a source of protein and 3-MST. Perhaps, these rhinoceros larva could be introduced on farmland with contaminated soil whereby they will process the dead roots and plants into soil thereby providing more space and manure for plants to grow healthy.
Thesis Overview
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